EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A partial atomic structure for the flagellar hook of Salmonella typhimurium.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 102, Issue 4, Page 1023-1028, Year 2005
掲載日	2005年1月25日
著者	Tanvir R Shaikh / Dennis R Thomas / James Z Chen / Fadel A Samatey / Hideyuki Matsunami / Katsumi Imada / Keiichi Namba / David J Derosier /
PubMed 要旨	The axial proteins of the bacterial flagellum function as a drive shaft, universal joint, and propeller driven by the flagellar rotary motor; they also form the putative protein export channel. The N- ...The axial proteins of the bacterial flagellum function as a drive shaft, universal joint, and propeller driven by the flagellar rotary motor; they also form the putative protein export channel. The N- and C-terminal sequences of the eight axial proteins were predicted to form interlocking alpha-domains generating an axial tube. We report on an approximately 1-nm resolution map of the hook from Salmonella typhimurium, which reveals such a tube made from interdigitated, 1-nm rod-like densities similar to those seen in maps of the filament. Atomic models for the two outer domains of the hook subunit were docked into the corresponding outermost features of the map. The N and C termini of the hook subunit fragment are positioned next to each other and face toward the axis of the hook. The placement of these termini would permit the residues missing in the fragment to form the rod-like features that form the core domain of the hook. We also fit the hook atomic model to an approximately 2-nm resolution map of the hook from Caulobacter crescentus. The hook protein sequence from C. crescentus is largely homologous to that of S. typhimurium except for a large insertion (20 kDa). According to difference maps and our fitting, this insertion is found on the outer surface of the hook, consistent with our modeling of the hook.
リンク	Proc Natl Acad Sci U S A / PubMed:15657146 / PubMed Central
手法	EM (らせん対称) / EM (電子線結晶学)
解像度	9.0 Å
構造データ	1132 2bgy 2bgz EMDB-1132: A partial atomic structure for the flagellar hook of Salmonella typhimurium. PDB-2bgy: Fit of the x-ray structure of the baterial flagellar hook fragment flge31 into an EM map from the hook of Caulobacter crescentus. PDB-2bgz: ATOMIC MODEL OF THE BACTERIAL FLAGELLAR BASED ON DOCKING AN X-RAY DERIVED HOOK STRUCTURE INTO AN EM MAP. 手法: EM (らせん対称) / 解像度: 9.0 Å
由来	Salmonella enterica subsp. enterica serovar Typhimurium (サルモネラ菌) salmonella typhimurium (サルモネラ菌)
キーワード	MOTOR PROTEIN (モータータンパク質) / BACTERIAL MOTILITY / FLAGELLAR HOOK / FLAGELLUM (鞭毛) / BACTERIAL MOTIILITY / BACTERIAL FLAGELLAR HOOK