EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The Hantavirus Surface Glycoprotein Lattice and Its Fusion Control Mechanism.
ジャーナル・号・ページ	Cell, Vol. 183, Issue 2, Page 442-456.e16, Year 2020
掲載日	2020年10月15日
著者	Alexandra Serris / Robert Stass / Eduardo A Bignon / Nicolás A Muena / Jean-Claude Manuguerra / Rohit K Jangra / Sai Li / Kartik Chandran / Nicole D Tischler / Juha T Huiskonen / Felix A Rey / Pablo Guardado-Calvo /
PubMed 要旨	Hantaviruses are rodent-borne viruses causing serious zoonotic outbreaks worldwide for which no treatment is available. Hantavirus particles are pleomorphic and display a characteristic square ...Hantaviruses are rodent-borne viruses causing serious zoonotic outbreaks worldwide for which no treatment is available. Hantavirus particles are pleomorphic and display a characteristic square surface lattice. The envelope glycoproteins Gn and Gc form heterodimers that further assemble into tetrameric spikes, the lattice building blocks. The glycoproteins, which are the sole targets of neutralizing antibodies, drive virus entry via receptor-mediated endocytosis and endosomal membrane fusion. Here we describe the high-resolution X-ray structures of the heterodimer of Gc and the Gn head and of the homotetrameric Gn base. Docking them into an 11.4-Å-resolution cryoelectron tomography map of the hantavirus surface accounted for the complete extramembrane portion of the viral glycoprotein shell and allowed a detailed description of the surface organization of these pleomorphic virions. Our results, which further revealed a built-in mechanism controlling Gc membrane insertion for fusion, pave the way for immunogen design to protect against pathogenic hantaviruses.
リンク	Cell / PubMed:32937107 / PubMed Central
手法	EM (サブトモグラム平均) / X線回折
解像度	1.902 - 15.6 Å
構造データ	11236 6zjm EMDB-11236: Reconstruction of Tula virus surface glycoprotein lattice PDB-6zjm: Atomic model of Andes virus glycoprotein spike tetramer generated by fitting into a Tula virus reconstruction 手法: EM (サブトモグラム平均) / 解像度: 11.4 Å EMDB-4867: Sub-tomogram averaging of Tula virus glycoprotein spike 手法: EM (サブトモグラム平均) / 解像度: 15.6 Å PDB-6y5f: Crystal structure of the envelope glycoprotein prefusion complex of Andes virus - Mutant H953F 手法: X-RAY DIFFRACTION / 解像度: 3.2 Å PDB-6y5w: Crystal structure of the envelope glycoprotein complex of Andes virus in a near postfusion conformation 手法: X-RAY DIFFRACTION / 解像度: 2.55 Å PDB-6y62: Crystal structure of the envelope glycoprotein complex of Maporal virus in a prefusion conformation 手法: X-RAY DIFFRACTION / 解像度: 2.2 Å PDB-6y68: Structure of Maporal virus envelope glycoprotein Gc in postfusion conformation 手法: X-RAY DIFFRACTION / 解像度: 2.4 Å PDB-6y6p: Structure of Hantaan virus envelope glycoprotein Gn 手法: X-RAY DIFFRACTION / 解像度: 1.938 Å PDB-6y6q: Structure of Andes virus envelope glycoprotein Gc in postfusion conformation 手法: X-RAY DIFFRACTION / 解像度: 2.7 Å PDB-6yrb: Crystal structure of the tetramerization domain of the glycoprotein Gn (Andes virus) at pH 7.5 手法: X-RAY DIFFRACTION / 解像度: 2.351 Å PDB-6yrq: Crystal structure of the tetramerization domain of the glycoprotein Gn (Andes virus) at pH 4.6 手法: X-RAY DIFFRACTION / 解像度: 1.902 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-PO4: PHOSPHATE ION / ホスファ-ト / リン酸塩 ChemComp-HOH: WATER / 水 ChemComp-CD: Unknown entry ChemComp-SO4: SULFATE ION / 硫酸ジアニオン / 硫酸塩 ChemComp-IOD: IODIDE ION / ヨ-ジド / ヨウ化物 ChemComp-MPD: (4S)-2-METHYL-2,4-PENTANEDIOL / (S)-2-メチル-2,4-ペンタンジオ-ル / 沈殿剤*YM / 2-Methyl-2,4-pentanediol
由来	tula orthohantavirus (ウイルス) andes orthohantavirus (ウイルス) maporal virus (ウイルス) hantaan orthohantavirus (ウイルス) drosophila melanogaster (キイロショウジョウバエ)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / class-II fusion protein hantavirus bunyavirus prefusion complex / class-II fusion protein hantavirus bunyavirus / prefusion complex / postfusion conformation / Hantavirus (ハンタウイルス) / Tula / Andes / TULV / ANDV / glycoprotein (糖タンパク質) / lattice / spike / fusion protein (融合タンパク質)