EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Direct visualization of degradation microcompartments at the ER membrane.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 117, Issue 2, Page 1069-1080, Year 2020
掲載日	2020年1月14日
著者	Sahradha Albert / Wojciech Wietrzynski / Chia-Wei Lee / Miroslava Schaffer / Florian Beck / Jan M Schuller / Patrice A Salomé / Jürgen M Plitzko / Wolfgang Baumeister / Benjamin D Engel /
PubMed 要旨	To promote the biochemical reactions of life, cells can compartmentalize molecular interaction partners together within separated non-membrane-bound regions. It is unknown whether this strategy is ...To promote the biochemical reactions of life, cells can compartmentalize molecular interaction partners together within separated non-membrane-bound regions. It is unknown whether this strategy is used to facilitate protein degradation at specific locations within the cell. Leveraging in situ cryo-electron tomography to image the native molecular landscape of the unicellular alga , we discovered that the cytosolic protein degradation machinery is concentrated within ∼200-nm foci that contact specialized patches of endoplasmic reticulum (ER) membrane away from the ER-Golgi interface. These non-membrane-bound microcompartments exclude ribosomes and consist of a core of densely clustered 26S proteasomes surrounded by a loose cloud of Cdc48. Active proteasomes in the microcompartments directly engage with putative substrate at the ER membrane, a function canonically assigned to Cdc48. Live-cell fluorescence microscopy revealed that the proteasome clusters are dynamic, with frequent assembly and fusion events. We propose that the microcompartments perform ER-associated degradation, colocalizing the degradation machinery at specific ER hot spots to enable efficient protein quality control.
リンク	Proc Natl Acad Sci U S A / PubMed:31882451 / PubMed Central
手法	EM (トモグラフィー) / EM (サブトモグラム平均)
解像度	17.24 - 35.0 Å
構造データ	EMDB-10409: In situ cryo-electron tomogram from Chlamydomonas reinhardtii of a proteasome cluster at the endoplasmic reticulum 手法: EM (トモグラフィー) EMDB-10410: In situ subtomogram average of Chlamydomonas Cdc48 (C6 symmetry) 手法: EM (サブトモグラム平均) / 解像度: 32.8 Å EMDB-10411: In situ subtomogram average of Chlamydomonas Cdc48 (C1 symmetry) 手法: EM (サブトモグラム平均) / 解像度: 35.0 Å EMDB-3932: In situ subtomogram average of the Chlamydomonas double-capped 26S proteasome 手法: EM (サブトモグラム平均) / 解像度: 21.88 Å EMDB-3933: In situ subtomogram average of the Chlamydomonas single-capped 26S proteasome 手法: EM (サブトモグラム平均) / 解像度: 23.81 Å EMDB-3934: In situ subtomogram average of the Chlamydomonas ground state 26S proteasome 手法: EM (サブトモグラム平均) / 解像度: 17.24 Å EMDB-3935: In situ subtomogram average of the Chlamydomonas processing state 26S proteasome 手法: EM (サブトモグラム平均) / 解像度: 19.61 Å
由来	Chlamydomonas reinhardtii (クラミドモナス)