EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanism of completion of peptidyltransferase centre assembly in eukaryotes.
ジャーナル・号・ページ	Elife, Vol. 8, Year 2019
掲載日	2019年5月22日
著者	Vasileios Kargas / Pablo Castro-Hartmann / Norberto Escudero-Urquijo / Kyle Dent / Christine Hilcenko / Carolin Sailer / Gertrude Zisser / Maria J Marques-Carvalho / Simone Pellegrino / Leszek Wawiórka / Stefan Mv Freund / Jane L Wagstaff / Antonina Andreeva / Alexandre Faille / Edwin Chen / Florian Stengel / Helmut Bergler / Alan John Warren /
PubMed 要旨	During their final maturation in the cytoplasm, pre-60S ribosomal particles are converted to translation-competent large ribosomal subunits. Here, we present the mechanism of peptidyltransferase ...During their final maturation in the cytoplasm, pre-60S ribosomal particles are converted to translation-competent large ribosomal subunits. Here, we present the mechanism of peptidyltransferase centre (PTC) completion that explains how integration of the last ribosomal proteins is coupled to release of the nuclear export adaptor Nmd3. Single-particle cryo-EM reveals that eL40 recruitment stabilises helix 89 to form the uL16 binding site. The loading of uL16 unhooks helix 38 from Nmd3 to adopt its mature conformation. In turn, partial retraction of the L1 stalk is coupled to a conformational switch in Nmd3 that allows the uL16 P-site loop to fully accommodate into the PTC where it competes with Nmd3 for an overlapping binding site (base A2971). Our data reveal how the central functional site of the ribosome is sculpted and suggest how the formation of translation-competent 60S subunits is disrupted in leukaemia-associated ribosomopathies.
リンク	Elife / PubMed:31115337 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 3.9 Å
構造データ	EMDB-10039: Cryo-EM structures of Lsg1-TAP pre-60S ribosomal particles (State V - subclass 1) 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-10066: Cryo-EM structure of Lsg1-TAP pre-60S ribosomal particles (State I - subclass 1) 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-10067: Cryo-EM structure of Lsg1-TAP ribosomal subunit (State I - subclass 2) 手法: EM (単粒子) / 解像度: 3.4 Å 10068 6rzz EMDB-10068, PDB-6rzz: Cryo-EM structures of Lsg1-TAP pre-60S ribosomal particles 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-10070: Cryo-EM structure of Lsg1-TAP pre-60S ribosomal particles (State II - subclass 1) 手法: EM (単粒子) / 解像度: 3.6 Å 10071 6s05 EMDB-10071, PDB-6s05: Cryo-EM structures of Lsg1-TAP pre-60S ribosomal particles 手法: EM (単粒子) / 解像度: 3.9 Å 4560 6qik EMDB-4560, PDB-6qik: Cryo-EM structures of Lsg1-TAP pre-60S ribosomal particles 手法: EM (単粒子) / 解像度: 3.1 Å 4630 6qt0 EMDB-4630, PDB-6qt0: Cryo-EM structures of Lsg1-TAP pre-60S ribosomal particles 手法: EM (単粒子) / 解像度: 3.4 Å 4636 6qtz EMDB-4636, PDB-6qtz: Cryo-EM structures of Lsg1-TAP pre-60S ribosomal particles 手法: EM (単粒子) / 解像度: 3.5 Å 4884 6ri5 EMDB-4884, PDB-6ri5: Cryo-EM structures of Lsg1-TAP pre-60S ribosomal particles 手法: EM (単粒子) / 解像度: 3.3 Å
化合物	ChemComp-ZN: Unknown entry
由来	saccharomyces cerevisiae (パン酵母) Baker's yeast (パン酵母) Saccharomyces cerevisiae (出芽酵母)
キーワード	RIBOSOME (リボソーム) / 60S subunit / eIF6 / Nmd3 / Lsg1