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-Structure paper
タイトル | Ribosome interactions of aminoacyl-tRNA and elongation factor Tu in the codon-recognition complex. |
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ジャーナル・号・ページ | Nat Struct Biol, Vol. 9, Issue 11, Page 849-854, Year 2002 |
掲載日 | 2002年11月25日 |
著者 | Holger Stark / Marina V Rodnina / Hans-Joachim Wieden / Friedrich Zemlin / Wolfgang Wintermeyer / Marin van Heel / |
PubMed 要旨 | The mRNA codon in the ribosomal A-site is recognized by aminoacyl-tRNA (aa-tRNA) in a ternary complex with elongation factor Tu (EF-Tu) and GTP. Here we report the 13 A resolution three-dimensional ...The mRNA codon in the ribosomal A-site is recognized by aminoacyl-tRNA (aa-tRNA) in a ternary complex with elongation factor Tu (EF-Tu) and GTP. Here we report the 13 A resolution three-dimensional reconstruction determined by cryo-electron microscopy of the kirromycin-stalled codon-recognition complex. The structure of the ternary complex is distorted by binding of the tRNA anticodon arm in the decoding center. The aa-tRNA interacts with 16S rRNA, helix 69 of 23S rRNA and proteins S12 and L11, while the sarcin-ricin loop of 23S rRNA contacts domain 1 of EF-Tu near the nucleotide-binding pocket. These results provide a detailed snapshot view of an important functional state of the ribosome and suggest mechanisms of decoding and GTPase activation. |
リンク | Nat Struct Biol / PubMed:12379845 |
手法 | EM (単粒子) |
解像度 | 13.0 Å |
構造データ | |
由来 |
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キーワード | RIBOSOME (リボソーム) / 70S RIBOSOME (リボソーム) / LOW RESOLUTION MODEL TERNARY COMPLEX / EF-Tu (EF-Tu) |