EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The complete structure of the human TFIIH core complex.
ジャーナル・号・ページ	Elife, Vol. 8, Year 2019
掲載日	2019年3月12日
著者	Basil J Greber / Daniel B Toso / Jie Fang / Eva Nogales /
PubMed 要旨	Transcription factor IIH (TFIIH) is a heterodecameric protein complex critical for transcription initiation by RNA polymerase II and nucleotide excision DNA repair. The TFIIH core complex is ...Transcription factor IIH (TFIIH) is a heterodecameric protein complex critical for transcription initiation by RNA polymerase II and nucleotide excision DNA repair. The TFIIH core complex is sufficient for its repair functions and harbors the XPB and XPD DNA-dependent ATPase/helicase subunits, which are affected by human disease mutations. Transcription initiation additionally requires the CdK activating kinase subcomplex. Previous structural work has provided only partial insight into the architecture of TFIIH and its interactions within transcription pre-initiation complexes. Here, we present the complete structure of the human TFIIH core complex, determined by phase-plate cryo-electron microscopy at 3.7 Å resolution. The structure uncovers the molecular basis of TFIIH assembly, revealing how the recruitment of XPB by p52 depends on a pseudo-symmetric dimer of homologous domains in these two proteins. The structure also suggests a function for p62 in the regulation of XPD, and allows the mapping of previously unresolved human disease mutations.
リンク	Elife / PubMed:30860024 / PubMed Central
手法	EM (単粒子)
解像度	3.6 - 4.3 Å
構造データ	0452 6nmi EMDB-0452, PDB-6nmi: Cryo-EM structure of the human TFIIH core complex 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-0587: Cryo-EM structure of the human TFIIH core complex: Map sorted for the MAT1 RING domain. 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-0588: Cryo-EM structure of the human TFIIH core complex: Map classified for absence of the p62 BSD2 domain. 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-0589: Cryo-EM structure of the human TFIIH core complex: Map classified for presence of the p62 BSD2 domain. 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-0602: Cryo-EM structure of the human TFIIH core complex: Multibody-refined map, body 1 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-0603: Cryo-EM structure of the human TFIIH core complex: Multibody-refined map, body 2 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-0604: Cryo-EM structure of the human TFIIH core complex: Multibody-refined map, body 3 手法: EM (単粒子) / 解像度: 3.7 Å
化合物	ChemComp-SF4: IRON/SULFUR CLUSTER / 鉄・硫黄クラスター ChemComp-ZN: Unknown entry
由来	homo sapiens (ヒト) Human (ヒト)
キーワード	TRANSCRIPTION (転写 (生物学)) / DNA repair (DNA修復) / helicase (ヘリカーゼ) / multiprotein complex (タンパク質複合体)