EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Influenza hemagglutinin membrane anchor.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 115, Issue 40, Page 10112-10117, Year 2018
掲載日	2018年10月2日
著者	Donald J Benton / Andrea Nans / Lesley J Calder / Jack Turner / Ursula Neu / Yi Pu Lin / Esther Ketelaars / Nicole L Kallewaard / Davide Corti / Antonio Lanzavecchia / Steven J Gamblin / Peter B Rosenthal / John J Skehel /
PubMed 要旨	Viruses with membranes fuse them with cellular membranes, to transfer their genomes into cells at the beginning of infection. For Influenza virus, the membrane glycoprotein involved in fusion is the ...Viruses with membranes fuse them with cellular membranes, to transfer their genomes into cells at the beginning of infection. For Influenza virus, the membrane glycoprotein involved in fusion is the hemagglutinin (HA), the 3D structure of which is known from X-ray crystallographic studies. The soluble ectodomain fragments used in these studies lacked the "membrane anchor" portion of the molecule. Since this region has a role in membrane fusion, we have determined its structure by analyzing the intact, full-length molecule in a detergent micelle, using cryo-EM. We have also compared the structures of full-length HA-detergent micelles with full-length HA-Fab complex detergent micelles, to describe an infectivity-neutralizing monoclonal Fab that binds near the ectodomain membrane anchor junction. We determine a high-resolution HA structure which compares favorably in detail with the structure of the ectodomain seen by X-ray crystallography; we detect, clearly, all five carbohydrate side chains of HA; and we find that the ectodomain is joined to the membrane anchor by flexible, eight-residue-long, linkers. The linkers extend into the detergent micelle to join a central triple-helical structure that is a major component of the membrane anchor.
リンク	Proc Natl Acad Sci U S A / PubMed:30224494 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.87 - 4.2 Å
構造データ	0234 6hjn EMDB-0234, PDB-6hjn: Structure of Influenza Hemagglutinin ectodomain (A/duck/Alberta/35/76) 手法: EM (単粒子) / 解像度: 3.3 Å 0235 6hjp EMDB-0235, PDB-6hjp: Structure of Influenza Hemagglutinin ectodomain (A/duck/Alberta/35/76) in complex with FISW84 Fab Fragment 手法: EM (単粒子) / 解像度: 3.3 Å 0236 6hjq EMDB-0236, PDB-6hjq: Structure of Full-length Influenza Hemagglutinin (A/duck/Alberta/35/76) in complex with FISW84 Fab Fragment 手法: EM (単粒子) / 解像度: 4.1 Å 0237 6hjr EMDB-0237, PDB-6hjr: Structure of full-length Influenza Hemagglutinin with tilted transmembrane (A/duck/Alberta/35/76[H1N1]) 手法: EM (単粒子) / 解像度: 4.2 Å PDB-6hkg: Structure of FISW84 Fab Fragment 手法: X-RAY DIFFRACTION / 解像度: 1.87 Å
化合物	ChemComp-UPL: UNKNOWN BRANCHED FRAGMENT OF PHOSPHOLIPID ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-SO4: SULFATE ION / 硫酸ジアニオン / 硫酸塩 ChemComp-HOH: WATER / 水
由来	influenza a virus (strain a/duck/alberta/35/1976 h1n1) (A型インフルエンザウイルス) homo sapiens (ヒト)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / Influenza virus (オルトミクソウイルス科) / Hemagglutinin (ヘマグルチニン) / Membrane protein (膜タンパク質) / Membrane fusion / IMMUNE SYSTEM (免疫系)