+検索条件
-Structure paper
タイトル | A novel mechanism for small heat shock proteins to function as molecular chaperones. |
---|---|
ジャーナル・号・ページ | Sci Rep, Vol. 5, Page 8811, Year 2015 |
掲載日 | 2015年3月6日 |
著者 | Kaiming Zhang / Anastasia N Ezemaduka / Zhao Wang / Hongli Hu / Xiaodong Shi / Chuang Liu / Xinping Lu / Xinmiao Fu / Zengyi Chang / Chang-Cheng Yin / |
PubMed 要旨 | Small heat shock proteins (sHSPs) are molecular chaperones ubiquitously present in all forms of life, but their function mechanisms remain controversial. Here we show by cryo-electron microscopy and ...Small heat shock proteins (sHSPs) are molecular chaperones ubiquitously present in all forms of life, but their function mechanisms remain controversial. Here we show by cryo-electron microscopy and single particle 3D reconstruction that, at the low temperatures (4-25°C), CeHSP17 (a sHSP from Caenorhabditis elegans) exists as a 24-subunit spherical oligomer with tetrahedral symmetry. Our studies demonstrate that CeHSP17 forms large sheet-like super-molecular assemblies (SMAs) at the high temperatures (45-60°C), and such SMAs are apparently the form that exhibits chaperone-like activity. Our findings suggest a novel molecular mechanism for sHSPs to function as molecular chaperones. |
リンク | Sci Rep / PubMed:25744691 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 14.5 Å |
構造データ | EMDB-2800: |
由来 |
|