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Structure paper

タイトル	Structure of the voltage-gated calcium channel Ca(v)1.1 at 3.6 Å resolution.
ジャーナル・号・ページ	Nature, Vol. 537, Issue 7619, Page 191-196, Year 2016
掲載日	2016年9月8日
著者	Jianping Wu / Zhen Yan / Zhangqiang Li / Xingyang Qian / Shan Lu / Mengqiu Dong / Qiang Zhou / Nieng Yan /
PubMed 要旨	The voltage-gated calcium (Ca) channels convert membrane electrical signals to intracellular Ca-mediated events. Among the ten subtypes of Ca channel in mammals, Ca1.1 is specified for the excitation- ...The voltage-gated calcium (Ca) channels convert membrane electrical signals to intracellular Ca-mediated events. Among the ten subtypes of Ca channel in mammals, Ca1.1 is specified for the excitation-contraction coupling of skeletal muscles. Here we present the cryo-electron microscopy structure of the rabbit Ca1.1 complex at a nominal resolution of 3.6 Å. The inner gate of the ion-conducting α1-subunit is closed and all four voltage-sensing domains adopt an 'up' conformation, suggesting a potentially inactivated state. The extended extracellular loops of the pore domain, which are stabilized by multiple disulfide bonds, form a windowed dome above the selectivity filter. One side of the dome provides the docking site for the α2δ-1-subunit, while the other side may attract cations through its negative surface potential. The intracellular I-II and III-IV linker helices interact with the β-subunit and the carboxy-terminal domain of α1, respectively. Classification of the particles yielded two additional reconstructions that reveal pronounced displacement of β and adjacent elements in α1. The atomic model of the Ca1.1 complex establishes a foundation for mechanistic understanding of excitation-contraction coupling and provides a three-dimensional template for molecular interpretations of the functions and disease mechanisms of Ca and Na channels.
リンク	Nature / PubMed:27580036
手法	EM (単粒子)
解像度	3.6 - 3.9 Å
構造データ	9513 5gjv EMDB-9513: Cryo-EM map of the rabbit voltage-gated calcium channel Cav1.1 at 3.6 angstrom resolution PDB-5gjv: Structure of the mammalian voltage-gated calcium channel Cav1.1 complex at near atomic resolution 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-9514: ClassIa map of the rabbit voltage-gated calcium channel Cav1.1 手法: EM (単粒子) / 解像度: 3.9 Å 9515 5gjw EMDB-9515: ClassII map of the rabbit voltage-gated calcium channel Cav1.1 PDB-5gjw: Structure of the mammalian voltage-gated calcium channel Cav1.1 complex for ClassII map 手法: EM (単粒子) / 解像度: 3.9 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM / ホスファチジルコリン ChemComp-CA*: Unknown entry / カルシウムジカチオン
由来	oryctolagus cuniculus (ウサギ)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / complex / channel