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TitleAcetylation regulates the oligomerization state and activity of RNase J, the Helicobacter pylori major ribonuclease.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 8072, Year 2023
Publish dateDec 6, 2023
AuthorsAlejandro Tejada-Arranz / Aleksei Lulla / Maxime Bouilloux-Lafont / Evelyne Turlin / Xue-Yuan Pei / Thibaut Douché / Mariette Matondo / Allison H Williams / Bertrand Raynal / Ben F Luisi / Hilde De Reuse /
PubMed AbstractIn the gastric pathogen Helicobacter pylori, post-transcriptional regulation relies strongly on the activity of the essential ribonuclease RNase J. Here, we elucidated the crystal and cryo-EM ...In the gastric pathogen Helicobacter pylori, post-transcriptional regulation relies strongly on the activity of the essential ribonuclease RNase J. Here, we elucidated the crystal and cryo-EM structures of RNase J and determined that it assembles into dimers and tetramers in vitro. We found that RNase J extracted from H. pylori is acetylated on multiple lysine residues. Alanine substitution of several of these residues impacts on H. pylori morphology, and thus on RNase J function in vivo. Mutations of Lysine 649 modulates RNase J oligomerization in vitro, which in turn influences ribonuclease activity in vitro. Our structural analyses of RNase J reveal loops that gate access to the active site and rationalizes how acetylation state of K649 can influence activity. We propose acetylation as a regulatory level controlling the activity of RNase J and its potential cooperation with other enzymes of RNA metabolism in H. pylori.
External linksNat Commun / PubMed:38057323 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.75 - 4.1 Å
Structure data

16647

8cgl

EMDB-16647, PDB-8cgl:
Cryo-EM structure of RNase J from Helicobacter pylori
Method: EM (single particle) / Resolution: 4.1 Å

PDB-7pcr:
Helicobacter pylori RNase J
Method: X-RAY DIFFRACTION / Resolution: 2.75 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • Helicobacter pylori (bacteria)
  • helicobacter pylori b128 (bacteria)
  • helicobacter pylori 26695 (bacteria)
KeywordsHYDROLASE / RNase J / Helicobacter pylori / RNA metabolism / RNA BINDING PROTEIN / ribonuclease / degradosome

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