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TitleIntrinsically disordered CsoS2 acts as a general molecular thread for α-carboxysome shell assembly.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 5512, Year 2023
Publish dateSep 7, 2023
AuthorsTao Ni / Qiuyao Jiang / Pei Cing Ng / Juan Shen / Hao Dou / Yanan Zhu / Julika Radecke / Gregory F Dykes / Fang Huang / Lu-Ning Liu / Peijun Zhang /
PubMed AbstractCarboxysomes are a paradigm of self-assembling proteinaceous organelles found in nature, offering compartmentalisation of enzymes and pathways to enhance carbon fixation. In α-carboxysomes, the ...Carboxysomes are a paradigm of self-assembling proteinaceous organelles found in nature, offering compartmentalisation of enzymes and pathways to enhance carbon fixation. In α-carboxysomes, the disordered linker protein CsoS2 plays an essential role in carboxysome assembly and Rubisco encapsulation. Its mechanism of action, however, is not fully understood. Here we synthetically engineer α-carboxysome shells using minimal shell components and determine cryoEM structures of these to decipher the principle of shell assembly and encapsulation. The structures reveal that the intrinsically disordered CsoS2 C-terminus is well-structured and acts as a universal "molecular thread" stitching through multiple shell protein interfaces. We further uncover in CsoS2 a highly conserved repetitive key interaction motif, [IV]TG, which is critical to the shell assembly and architecture. Our study provides a general mechanism for the CsoS2-governed carboxysome shell assembly and cargo encapsulation and further advances synthetic engineering of carboxysomes for diverse biotechnological applications.
External linksNat Commun / PubMed:37679318 / PubMed Central
MethodsEM (single particle)
Resolution1.86 - 3.54 Å
Structure data

EMDB-15595: cryo-EM structure of carboxysomal mini-shell: oblate structure from C1 construct (T=7 Q=6)
Method: EM (single particle) / Resolution: 3.17 Å

EMDB-15611: cryo-EM structure of carboxysomal mini-shell: icosahedral assembly from C2 construct (T=4)
Method: EM (single particle) / Resolution: 2.26 Å

EMDB-15719: cryo-EM structure of carboxysome mini-shell: icosahedral structure from C1 construct (T=4)
Method: EM (single particle) / Resolution: 2.02 Å

EMDB-15720: cryo-EM structure of carboxysome mini-shell: icosahedral structure from C1 construct (T=7)
Method: EM (single particle) / Resolution: 3.29 Å

EMDB-15722: cryo-EM structure of carboxysome mini-shell: icosahedral structure from C1 construct (T=9)
Method: EM (single particle) / Resolution: 2.04 Å

EMDB-15723: cryo-EM structure of carboxysomal mini-shell: prolate structure from C1 construct (T=4 Q=6)
Method: EM (single particle) / Resolution: 2.65 Å

EMDB-15724: cryo-EM structure of carboxysomal mini-shell: prolate structure from C1 construct (T=4 Q=6) form 2
Method: EM (single particle) / Resolution: 2.69 Å

EMDB-15758: cryo-EM structure of carboxysomal mini-shell: icosahedral assembly from C3 construct (T=4)
Method: EM (single particle) / Resolution: 2.39 Å

EMDB-15759: cryo-EM structure of carboxysomal mini-shell: icosahedral assembly from C3 construct (T=4-P)
Method: EM (single particle) / Resolution: 3.28 Å

EMDB-15760: cryo-EM structure of carboxysomal mini-shell: icosahedral assembly from C3 construct (T=3)
Method: EM (single particle) / Resolution: 2.31 Å

EMDB-15761: cryo-EM structure of carboxysomal mini-shell: icosahedral assembly from C3 construct (T=3-P)
Method: EM (single particle) / Resolution: 2.68 Å

EMDB-15762: cryo-EM structure of carboxysomal mini-shell: icosahedral assembly from C1 ITG mutant construct (T=4)
Method: EM (single particle) / Resolution: 2.65 Å

EMDB-15792: cryo-EM structure of carboxysomal mini-shell: icosahedral assembly from CsoS4A-1A (T=4)
Method: EM (single particle) / Resolution: 3.54 Å

15798

8b0y

EMDB-15798, PDB-8b0y:
cryo-EM structure of carboxysomal mini-shell: icosahedral assembly from CsoS4A/1A co-expression (T = 3)
Method: EM (single particle) / Resolution: 2.79 Å

15799

8b11

EMDB-15799, PDB-8b11:
cryo-EM structure of carboxysomal mini-shell: icosahedral assembly from CsoS4A/1A and CsoS2 co-expression (T = 4)
Method: EM (single particle) / Resolution: 2.52 Å

15801

8b12

EMDB-15801, PDB-8b12:
cryo-EM structure of carboxysomal mini-shell: icosahedral assembly from CsoS4A/1A and CsoS2 co-expression (T = 9)
Method: EM (single particle) / Resolution: 1.86 Å

EMDB-15834: cryo-EM structure of carboxysomal mini-shell: icosahedral assembly from C1 ITG mutant construct (T=3)
Method: EM (single particle) / Resolution: 2.22 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • halothiobacillus neapolitanus (bacteria)
KeywordsSTRUCTURAL PROTEIN / carboxysome / shell / icosahedral symmetry

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