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Structure paper

TitleStructures of channelrhodopsin paralogs in peptidiscs explain their contrasting K and Na selectivities.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 4365, Year 2023
Publish dateJul 20, 2023
AuthorsTakefumi Morizumi / Kyumhyuk Kim / Hai Li / Elena G Govorunova / Oleg A Sineshchekov / Yumei Wang / Lei Zheng / Éva Bertalan / Ana-Nicoleta Bondar / Azam Askari / Leonid S Brown / John L Spudich / Oliver P Ernst /
PubMed AbstractKalium channelrhodopsin 1 from Hyphochytrium catenoides (HcKCR1) is a light-gated channel used for optogenetic silencing of mammalian neurons. It selects K over Na in the absence of the canonical ...Kalium channelrhodopsin 1 from Hyphochytrium catenoides (HcKCR1) is a light-gated channel used for optogenetic silencing of mammalian neurons. It selects K over Na in the absence of the canonical tetrameric K selectivity filter found universally in voltage- and ligand-gated channels. The genome of H. catenoides also encodes a highly homologous cation channelrhodopsin (HcCCR), a Na channel with >100-fold larger Na to K permeability ratio. Here, we use cryo-electron microscopy to determine atomic structures of these two channels embedded in peptidiscs to elucidate structural foundations of their dramatically different cation selectivity. Together with structure-guided mutagenesis, we show that K versus Na selectivity is determined at two distinct sites on the putative ion conduction pathway: in a patch of critical residues in the intracellular segment (Leu69/Phe69, Ile73/Ser73 and Asp116) and within a cluster of aromatic residues in the extracellular segment (primarily, Trp102 and Tyr222). The two filters are on the opposite sides of the photoactive site involved in channel gating.
External linksNat Commun / PubMed:37474513 / PubMed Central
MethodsEM (single particle)
Resolution2.84 - 2.88 Å
Structure data

40062

8gi8

EMDB-40062, PDB-8gi8:
Kalium channelrhodopsin 1 from Hyphochytrium catenoides (HcKCR1) embedded in peptidisc
Method: EM (single particle) / Resolution: 2.88 Å

40063

8gi9

EMDB-40063, PDB-8gi9:
Cation channelrhodopsin from Hyphochytrium catenoides (HcCCR) embedded in peptidisc
Method: EM (single particle) / Resolution: 2.84 Å

Chemicals

ChemComp-RET:
RETINAL / Retinal

ChemComp-NA:
Unknown entry

ChemComp-CLR:
CHOLESTEROL / Cholesterol

ChemComp-PEE:
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, phospholipid*YM / Discrete optimized protein energy

ChemComp-HOH:
WATER / Water

Source
  • hyphochytrium catenoides (eukaryote)
KeywordsTRANSPORT PROTEIN / Retinal Protein / Channelrhodopsin / Cation channel / Peptidisc / Optogenetics

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