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TitleA mechanical-coupling mechanism in OSCA/TMEM63 channel mechanosensitivity.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 3943, Year 2023
Publish dateJul 4, 2023
AuthorsMingfeng Zhang / Yuanyue Shan / Charles D Cox / Duanqing Pei /
PubMed AbstractMechanosensitive (MS) ion channels are a ubiquitous type of molecular force sensor sensing forces from the surrounding bilayer. The profound structural diversity in these channels suggests that the ...Mechanosensitive (MS) ion channels are a ubiquitous type of molecular force sensor sensing forces from the surrounding bilayer. The profound structural diversity in these channels suggests that the molecular mechanisms of force sensing follow unique structural blueprints. Here we determine the structures of plant and mammalian OSCA/TMEM63 proteins, allowing us to identify essential elements for mechanotransduction and propose roles for putative bound lipids in OSCA/TMEM63 mechanosensation. Briefly, the central cavity created by the dimer interface couples each subunit and modulates dimeric OSCA/TMEM63 channel mechanosensitivity through the modulating lipids while the cytosolic side of the pore is gated by a plug lipid that prevents the ion permeation. Our results suggest that the gating mechanism of OSCA/TMEM63 channels may combine structural aspects of the 'lipid-gated' mechanism of MscS and TRAAK channels and the calcium-induced gating mechanism of the TMEM16 family, which may provide insights into the structural rearrangements of TMEM16/TMC superfamilies.
External linksNat Commun / PubMed:37402734 / PubMed Central
MethodsEM (single particle)
Resolution2.5 - 3.5 Å
Structure data

34209

8grn

EMDB-34209, PDB-8grn:
AtOSCA1.1 extended state
Method: EM (single particle) / Resolution: 2.5 Å

34210

8gro

EMDB-34210, PDB-8gro:
AtOSCA3.1 contracted state
Method: EM (single particle) / Resolution: 3.5 Å

34214

8grs

EMDB-34214, PDB-8grs:
human TMEM63A
Method: EM (single particle) / Resolution: 3.3 Å

34237

8gso

EMDB-34237, PDB-8gso:
AtOSCA3.1 channel extended state
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-LPC:
[1-MYRISTOYL-GLYCEROL-3-YL]PHOSPHONYLCHOLINE

ChemComp-POV:
(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / phospholipid*YM / POPC

Source
  • Arabidopsis (plant)
  • arabidopsis thaliana (thale cress)
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / channel

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