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TitleStructural basis of TFIIIC-dependent RNA polymerase III transcription initiation.
Journal, issue, pagesMol Cell, Vol. 83, Issue 15, Page 2641-22652.e7, Year 2023
Publish dateAug 3, 2023
AuthorsAnna Talyzina / Yan Han / Chiranjib Banerjee / Susan Fishbain / Alexis Reyes / Reza Vafabakhsh / Yuan He /
PubMed AbstractRNA polymerase III (Pol III) is responsible for transcribing 5S ribosomal RNA (5S rRNA), tRNAs, and other short non-coding RNAs. Its recruitment to the 5S rRNA promoter requires transcription factors ...RNA polymerase III (Pol III) is responsible for transcribing 5S ribosomal RNA (5S rRNA), tRNAs, and other short non-coding RNAs. Its recruitment to the 5S rRNA promoter requires transcription factors TFIIIA, TFIIIC, and TFIIIB. Here, we use cryoelectron microscopy (cryo-EM) to visualize the S. cerevisiae complex of TFIIIA and TFIIIC bound to the promoter. Gene-specific factor TFIIIA interacts with DNA and acts as an adaptor for TFIIIC-promoter interactions. We also visualize DNA binding of TFIIIB subunits, Brf1 and TBP (TATA-box binding protein), which results in the full-length 5S rRNA gene wrapping around the complex. Our smFRET study reveals that the DNA within the complex undergoes both sharp bending and partial dissociation on a slow timescale, consistent with the model predicted from our cryo-EM results. Our findings provide new insights into the transcription initiation complex assembly on the 5S rRNA promoter and allow us to directly compare Pol III and Pol II transcription adaptations.
External linksMol Cell / PubMed:37402369 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 7.14 Å
Structure data

29071

8ffz

EMDB-29071, PDB-8ffz:
TFIIIA-TFIIIC-Brf1-TBP complex bound to 5S rRNA gene
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-29356: Focused refinement on the Brf1-TBP-DNA within TFIIIA-TFIIIC-Brf1-TBP complex bound to 5S rRNA gene
Method: EM (single particle) / Resolution: 7.14 Å

EMDB-29358: TFIIIA-TFIIIC complex bound to 5S rRNA gene
Method: EM (single particle) / Resolution: 6.62 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces cerevisiae s288c (yeast)
KeywordsTRANSCRIPTION/DNA / transcription factor / TRANSCRIPTION / TRANSCRIPTION-DNA complex

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