EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Lipid nanodiscs as a template for high-resolution cryo-EM structures of peripheral membrane proteins.
ジャーナル・号・ページ	J Struct Biol, Vol. 215, Issue 3, Page 107989, Year 2023
掲載日	2023年6月24日
著者	Kevin S Cannon / Reta D Sarsam / Tanita Tedamrongwanish / Kevin Zhang / Richard W Baker /
PubMed 要旨	Peripheral membrane proteins are ubiquitous throughout cell biology and are required for a variety of cellular processes such as signal transduction, membrane trafficking, and autophagy. Transient ...Peripheral membrane proteins are ubiquitous throughout cell biology and are required for a variety of cellular processes such as signal transduction, membrane trafficking, and autophagy. Transient binding to the membrane has a profound impact on protein function, serving to induce conformational changes and alter biochemical and biophysical parameters by increasing the local concentration of factors and restricting diffusion to two dimensions. Despite the centrality of the membrane in serving as a template for cell biology, there are few reported high-resolution structures of peripheral membrane proteins bound to the membrane. We analyzed the utility of lipid nanodiscs to serve as a template for cryo-EM analysis of peripheral membrane proteins. We tested a variety of nanodiscs and we report a 3.3 Å structure of the AP2 clathrin adaptor complex bound to a 17-nm nanodisc, with sufficient resolution to visualize a bound lipid head group. Our data demonstrate that lipid nanodiscs are amenable to high-resolution structure determination of peripheral membrane proteins and provide a framework for extending this analysis to other systems.
リンク	J Struct Biol / PubMed:37364761
手法	EM (単粒子)
解像度	3.3 - 6.8 Å
構造データ	EMDB-16803: AP2 bound to MSP1 nanodisc with Tgn38 cargo peptide 手法: EM (単粒子) / 解像度: 4.9 Å EMDB-16804: AP2 bound to MSP1E3D1 nanodisc with Tgn38 cargo peptide 手法: EM (単粒子) / 解像度: 6.8 Å EMDB-29977: AP2 bound to an MSP1 nanodisc 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-40034: Structure of AP2 bound to MSP1E3D1 nanodisc 手法: EM (単粒子) / 解像度: 5.4 Å EMDB-40035: Structure of AP2 bound to MSP2N2 nanodisc 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-40963: AP2 bound to MSP2N2 nanodisc with Tgn38 cargo peptide; focused refinement 1 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-40964: AP2 bound to MSP2N2 nanodisc with Tgn38 cargo peptide; focused refinement 2 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-40965: AP2 bound to MSP2N2 nanodisc with Tgn38 cargo peptide; focused refinement 3 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-40966: AP2 bound to MSP2N2 nanodisc with Tgn38 cargo peptide; consensus refinement 手法: EM (単粒子) / 解像度: 3.4 Å 40973 8t1o EMDB-40973, PDB-8t1o: AP2 bound to MSP2N2 nanodisc with Tgn38 cargo peptide; composite map 手法: EM (単粒子) / 解像度: 3.3 Å
化合物	ChemComp-PIO: [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate / 1-O-(1-O,2-O-ジオクタノイル-L-グリセロ-3-ホスホ)-D-myo-イノシト-ル4,5-ビスりん酸
由来	mus musculus (ハツカネズミ) rattus norvegicus (ドブネズミ) Homo sapiens (ヒト)
キーワード	ENDOCYTOSIS (エンドサイトーシス) / Clathrin-mediated endocytosis; peripheral membrane protein