EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Lesion recognition by XPC, TFIIH and XPA in DNA excision repair.
ジャーナル・号・ページ	Nature, Vol. 617, Issue 7959, Page 170-175, Year 2023
掲載日	2023年4月19日
著者	Jinseok Kim / Chia-Lung Li / Xuemin Chen / Yanxiang Cui / Filip M Golebiowski / Huaibin Wang / Fumio Hanaoka / Kaoru Sugasawa / Wei Yang /
PubMed 要旨	Nucleotide excision repair removes DNA lesions caused by ultraviolet light, cisplatin-like compounds and bulky adducts. After initial recognition by XPC in global genome repair or a stalled RNA ...Nucleotide excision repair removes DNA lesions caused by ultraviolet light, cisplatin-like compounds and bulky adducts. After initial recognition by XPC in global genome repair or a stalled RNA polymerase in transcription-coupled repair, damaged DNA is transferred to the seven-subunit TFIIH core complex (Core7) for verification and dual incisions by the XPF and XPG nucleases. Structures capturing lesion recognition by the yeast XPC homologue Rad4 and TFIIH in transcription initiation or DNA repair have been separately reported. How two different lesion recognition pathways converge and how the XPB and XPD helicases of Core7 move the DNA lesion for verification are unclear. Here we report on structures revealing DNA lesion recognition by human XPC and DNA lesion hand-off from XPC to Core7 and XPA. XPA, which binds between XPB and XPD, kinks the DNA duplex and shifts XPC and the DNA lesion by nearly a helical turn relative to Core7. The DNA lesion is thus positioned outside of Core7, as would occur with RNA polymerase. XPB and XPD, which track the lesion-containing strand but translocate DNA in opposite directions, push and pull the lesion-containing strand into XPD for verification.
リンク	Nature / PubMed:37076618 / PubMed Central
手法	EM (単粒子)
解像度	3.3 - 7.1 Å
構造データ	27996 8ebs EMDB-27996, PDB-8ebs: Initial DNA-lesion (Cy5) binding by XPC and TFIIH 手法: EM (単粒子) / 解像度: 4.0 Å 27997 8ebt EMDB-27997, PDB-8ebt: XPA repositioning Core7 of TFIIH relative to XPC-DNA lesion (Cy5) 手法: EM (単粒子) / 解像度: 3.9 Å 27998 8ebu EMDB-27998, PDB-8ebu: XPC release from Core7-XPA-DNA (Cy5) 手法: EM (単粒子) / 解像度: 3.3 Å 27999 8ebv EMDB-27999, PDB-8ebv: Initial DNA-lesion (AP) binding by XPC and TFIIH complex 1 手法: EM (単粒子) / 解像度: 7.1 Å 28000 8ebw EMDB-28000, PDB-8ebw: Initial DNA-lesion (AP) binding by XPC and TFIIH complex2 手法: EM (単粒子) / 解像度: 5.6 Å 28001 8ebx EMDB-28001, PDB-8ebx: XPA repositioning Core7 of TFIIH relative to XPC-DNA lesion (AP) 手法: EM (単粒子) / 解像度: 3.6 Å 28002 8eby EMDB-28002, PDB-8eby: XPC release from Core7-XPA-DNA (AP) 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-29673: Bulky DNA lesion recognition complex 3 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-29674: Initial DNA-lesion (Cy5) binding by XPC and TFIIH focused 手法: EM (単粒子) / 解像度: 4.2 Å
化合物	ChemComp-SF4: IRON/SULFUR CLUSTER / 鉄・硫黄クラスター ChemComp-ZN: Unknown entry ChemComp-CA: Unknown entry / カルシウムジカチオン
由来	homo sapiens (ヒト) synthetic construct (人工物)
キーワード	DNA BINDING PROTEIN/DNA / Protein-DNA complex (デオキシリボ核酸) / DNA BINDING PROTEIN-DNA complex