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TitleThe translating bacterial ribosome at 1.55 Å resolution generated by cryo-EM imaging services.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 1095, Year 2023
Publish dateFeb 25, 2023
AuthorsSimon A Fromm / Kate M O'Connor / Michael Purdy / Pramod R Bhatt / Gary Loughran / John F Atkins / Ahmad Jomaa / Simone Mattei /
PubMed AbstractOur understanding of protein synthesis has been conceptualised around the structure and function of the bacterial ribosome. This complex macromolecular machine is the target of important ...Our understanding of protein synthesis has been conceptualised around the structure and function of the bacterial ribosome. This complex macromolecular machine is the target of important antimicrobial drugs, an integral line of defence against infectious diseases. Here, we describe how open access to cryo-electron microscopy facilities combined with bespoke user support enabled structural determination of the translating ribosome from Escherichia coli at 1.55 Å resolution. The obtained structures allow for direct determination of the rRNA sequence to identify ribosome polymorphism sites in the E. coli strain used in this study and enable interpretation of the ribosomal active and peripheral sites at unprecedented resolution. This includes scarcely populated chimeric hybrid states of the ribosome engaged in several tRNA translocation steps resolved at ~2 Å resolution. The current map not only improves our understanding of protein synthesis but also allows for more precise structure-based drug design of antibiotics to tackle rising bacterial resistance.
External linksNat Commun / PubMed:36841832 / PubMed Central
MethodsEM (single particle)
Resolution1.55 - 2.2 Å
Structure data

15793

8b0x

EMDB-15793, PDB-8b0x:
Translating 70S ribosome in the unrotated state (P and E, tRNAs)
Method: EM (single particle) / Resolution: 1.55 Å

EMDB-15794: Translating 70S ribosome in the rotated state (A/A and P/E tRNAs)
Method: EM (single particle) / Resolution: 2.1 Å

EMDB-15795: Translating 70S ribosome in the rotated state (A/P and P/E tRNAs)
Method: EM (single particle) / Resolution: 2.1 Å

EMDB-15796: Translating 70S ribosome in the unrotated state (A and P tRNAs)
Method: EM (single particle) / Resolution: 2.2 Å

EMDB-15797: Translating 70S ribosome in the unrotated state (A,P and E tRNAs)
Method: EM (single particle) / Resolution: 1.67 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER / Water

Source
  • escherichia coli b (bacteria)
KeywordsRIBOSOME / 70S / bacterial / translation / high-resolution

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