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TitleInsights into substrate coordination and glycosyl transfer of poplar cellulose synthase-8.
Journal, issue, pagesbioRxiv, Year 2023
Publish dateFeb 7, 2023
AuthorsPreeti Verma / Albert L Kwansa / Ruoya Ho / Yaroslava G Yingling / Jochen Zimmer /
PubMed AbstractCellulose is an abundant cell wall component of land plants. It is synthesized from UDP-activated glucose molecules by cellulose synthase, a membrane-integrated processive glycosyltransferase. ...Cellulose is an abundant cell wall component of land plants. It is synthesized from UDP-activated glucose molecules by cellulose synthase, a membrane-integrated processive glycosyltransferase. Cellulose synthase couples the elongation of the cellulose polymer with its translocation across the plasma membrane. Here, we present substrate and product-bound cryogenic electron microscopy structures of the homotrimeric cellulose synthase isoform-8 (CesA8) from hybrid aspen (poplar). UDP-glucose binds to a conserved catalytic pocket adjacent to the entrance to a transmembrane channel. The substrate's glucosyl unit is coordinated by conserved residues of the glycosyltransferase domain and amphipathic interface helices. Site-directed mutagenesis of a conserved gating loop capping the active site reveals its critical function for catalytic activity. Molecular dynamics simulations reveal prolonged interactions of the gating loop with the substrate molecule, particularly across its central conserved region. These transient interactions likely facilitate the proper positioning of the substrate molecule for glycosyl transfer and cellulose translocation.
HIGHLIGHTS: Cryo-EM structures of substrate and product bound poplar cellulose synthase provide insights into substrate selectivitySite directed mutagenesis signifies a critical function of the gating loop for catalysisMolecular dynamics simulations support persistent gating loop - substrate interactionsGating loop helps in positioning the substrate molecule to facilitate cellulose elongationConserved cellulose synthesis substrate binding mechanism across the kingdoms.
External linksbioRxiv / PubMed:36798277 / PubMed Central
MethodsEM (single particle)
Resolution3.3 Å
Structure data

29678

8g27

EMDB-29678, PDB-8g27:
Hybrid aspen cellulose synthase-8 bound to UDP
Method: EM (single particle) / Resolution: 3.3 Å

29679

8g2j

EMDB-29679, PDB-8g2j:
Hybrid aspen cellulose synthase-8 bound to UDP-glucose
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-UDP:
URIDINE-5'-DIPHOSPHATE / UDP*YM / Uridine diphosphate

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER / Water

ChemComp-UPG:
URIDINE-5'-DIPHOSPHATE-GLUCOSE / Uridine diphosphate glucose

Source
  • populus tremula x populus tremuloides (plant)
  • populus tremula x p. tremuloides/amanita muscaria mixed est library (eukaryote)
KeywordsPLANT PROTEIN / Transferase / Cellulose / cell wall / UDP

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