EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Unravelling the regulation pathway of photosynthetic AB-GAPDH.
ジャーナル・号・ページ	Acta Crystallogr D Struct Biol, Vol. 78, Issue Pt 11, Page 1399-1411, Year 2022
掲載日	2022年11月1日
著者	Roberto Marotta / Alessandra Del Giudice / Libero Gurrieri / Silvia Fanti / Paolo Swuec / Luciano Galantini / Giuseppe Falini / Paolo Trost / Simona Fermani / Francesca Sparla /
PubMed 要旨	Oxygenic phototrophs perform carbon fixation through the Calvin-Benson cycle. Different mechanisms adjust the cycle and the light-harvesting reactions to rapid environmental changes. Photosynthetic ...Oxygenic phototrophs perform carbon fixation through the Calvin-Benson cycle. Different mechanisms adjust the cycle and the light-harvesting reactions to rapid environmental changes. Photosynthetic glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a key enzyme in the cycle. In land plants, different photosynthetic GAPDHs exist: the most abundant isoform is formed by AB heterotetramers and the least abundant by A homotetramers. Regardless of the subunit composition, GAPDH is the major consumer of photosynthetic NADPH and its activity is strictly regulated. While A-GAPDH is regulated by CP12, AB-GAPDH is autonomously regulated through the C-terminal extension (CTE) of its B subunits. Reversible inhibition of AB-GAPDH occurs via the oxidation of a cysteine pair located in the CTE and the substitution of NADP(H) with NAD(H) in the cofactor-binding site. These combined conditions lead to a change in the oligomerization state and enzyme inhibition. SEC-SAXS and single-particle cryo-EM analysis were applied to reveal the structural basis of this regulatory mechanism. Both approaches revealed that spinach (AB)-GAPDH oligomers with n = 1, 2, 4 and 5 co-exist in a dynamic system. B subunits mediate the contacts between adjacent tetramers in AB and AB oligomers. The CTE of each B subunit penetrates into the active site of a B subunit of the adjacent tetramer, which in turn moves its CTE in the opposite direction, effectively preventing the binding of the substrate 1,3-bisphosphoglycerate in the B subunits. The whole mechanism is made possible, and eventually controlled, by pyridine nucleotides. In fact, NAD(H), by removing NADP(H) from A subunits, allows the entrance of the CTE into the active site of the B subunit, hence stabilizing inhibited oligomers.
リンク	Acta Crystallogr D Struct Biol / PubMed:36322422
手法	EM (単粒子)
解像度	5.7 - 13.0 Å
構造データ	13824 7q53 EMDB-13824, PDB-7q53: Single Particle Cryo-EM structure of photosynthetic A2B2 glyceraldehyde 3-phosphate dehydrogenase from Spinacia oleracia 手法: EM (単粒子) / 解像度: 6.3 Å 13825 7q54 EMDB-13825, PDB-7q54: Single Particle Cryo-EM structure of photosynthetic A4B4-glyceraldehyde 3-phosphate dehydrogenase from Spinacia oleracia. 手法: EM (単粒子) / 解像度: 8.9 Å 13826 7q55 EMDB-13826, PDB-7q55: Single Particle Cryo-EM structure of photosynthetic A8B8 glyceraldehyde-3-phosphate dehydrogenase hexadecamer (major conformer) from Spinacia oleracia. 手法: EM (単粒子) / 解像度: 5.7 Å 13827 7q56 EMDB-13827, PDB-7q56: Single Particle Cryo-EM structure of photosynthetic A8B8 glyceraldehyde-3-phosphate dehydrogenase (minor conformer) from Spinacia oleracea. 手法: EM (単粒子) / 解像度: 7.1 Å 13828 7q57 EMDB-13828, PDB-7q57: Single Particle Cryo-EM structure of photosynthetic A10B10 glyceraldehyde-3-phospahte dehydrogenase from Spinacia oleracea. 手法: EM (単粒子) / 解像度: 13.0 Å
化合物	ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM / ニコチンアミドアデニンジヌクレオチド
由来	spinacia oleracea (ホウレンソウ) Spinach (ホウレンソウ)
キーワード	OXIDOREDUCTASE (酸化還元酵素) / Photosynthesis (光合成) / Calvin-Benson cycle (カルビン回路) / redox regulation / glyceraldehyde-3-phosphate dehydrogenase / glyceraldehyde 3-phosphate dehydrogenase