EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for HflXr-mediated antibiotic resistance in Listeria monocytogenes.
ジャーナル・号・ページ	Nucleic Acids Res, Vol. 50, Issue 19, Page 11285-11300, Year 2022
掲載日	2022年10月27日
著者	Timm O Koller / Kathryn J Turnbull / Karolis Vaitkevicius / Caillan Crowe-McAuliffe / Mohammad Roghanian / Ondřej Bulvas / Jose A Nakamoto / Tatsuaki Kurata / Christina Julius / Gemma C Atkinson / Jörgen Johansson / Vasili Hauryliuk / Daniel N Wilson /
PubMed 要旨	HflX is a ubiquitous bacterial GTPase that splits and recycles stressed ribosomes. In addition to HflX, Listeria monocytogenes contains a second HflX homolog, HflXr. Unlike HflX, HflXr confers ...HflX is a ubiquitous bacterial GTPase that splits and recycles stressed ribosomes. In addition to HflX, Listeria monocytogenes contains a second HflX homolog, HflXr. Unlike HflX, HflXr confers resistance to macrolide and lincosamide antibiotics by an experimentally unexplored mechanism. Here, we have determined cryo-EM structures of L. monocytogenes HflXr-50S and HflX-50S complexes as well as L. monocytogenes 70S ribosomes in the presence and absence of the lincosamide lincomycin. While the overall geometry of HflXr on the 50S subunit is similar to that of HflX, a loop within the N-terminal domain of HflXr, which is two amino acids longer than in HflX, reaches deeper into the peptidyltransferase center. Moreover, unlike HflX, the binding of HflXr induces conformational changes within adjacent rRNA nucleotides that would be incompatible with drug binding. These findings suggest that HflXr confers resistance using an allosteric ribosome protection mechanism, rather than by simply splitting and recycling antibiotic-stalled ribosomes.
リンク	Nucleic Acids Res / PubMed:36300626 / PubMed Central
手法	EM (単粒子)
解像度	2.1 - 3.1 Å
構造データ	15161 8a57 EMDB-15161, PDB-8a57: Cryo-EM structure of HflXr bound to the Listeria monocytogenes 50S ribosomal subunit. 手法: EM (単粒子) / 解像度: 2.3 Å 15175 8a5i EMDB-15175, PDB-8a5i: Cryo-EM structure of Lincomycin bound to the Listeria monocytogenes 50S ribosomal subunit. 手法: EM (単粒子) / 解像度: 2.3 Å 15204 8a63 EMDB-15204, PDB-8a63: Cryo-EM structure of Listeria monocytogenes 50S ribosomal subunit. 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-15670: Cryo-EM volume of HflX bound to the Listeria monocytogenes 50S ribosomal subunit 手法: EM (単粒子) / 解像度: 2.76 Å EMDB-15864: Cryo-EM map of lincomycin bound to the Listeria monocytogenes 50S ribosomal subunit. 手法: EM (単粒子) / 解像度: 2.1 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-SPD: SPERMIDINE / スペルミジン / スペルミジン ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-PUT: 1,4-DIAMINOBUTANE / プトレシン / プトレシン ChemComp-K: Unknown entry / カリウムカチオン ChemComp-GNP: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / Gpp(NH)p / GppNHp, GMPPNP, エネルギー貯蔵分子類似体YM / 5'-Guanylyl imidodiphosphate ChemComp-3QB: LINCOMYCIN / リンコマイシン / 抗生剤YM / リンコマイシン ChemComp-HOH: WATER / 水
由来	Listeria monocytogenes E (リステリア・モノサイトゲネス) listeria monocytogenes egd-e (リステリア・モノサイトゲネス)
キーワード	RIBOSOME (リボソーム) / Listeria monocytogenes (リステリア・モノサイトゲネス) / HflXr / 50S / antibiotic resistance (抗微生物薬耐性) / Lincomycin (リンコマイシン) / antibiotic (抗生物質)