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TitleCryo-electron microscopy unveils unique structural features of the human Kir2.1 channel.
Journal, issue, pagesSci Adv, Vol. 8, Issue 38, Page eabq8489, Year 2022
Publish dateSep 23, 2022
AuthorsCarlos A H Fernandes / Dania Zuniga / Charline Fagnen / Valérie Kugler / Rosa Scala / Gérard Péhau-Arnaudet / Renaud Wagner / David Perahia / Saïd Bendahhou / Catherine Vénien-Bryan /
PubMed AbstractWe present the first structure of the human Kir2.1 channel containing both transmembrane domain (TMD) and cytoplasmic domain (CTD). Kir2.1 channels are strongly inward-rectifying potassium channels ...We present the first structure of the human Kir2.1 channel containing both transmembrane domain (TMD) and cytoplasmic domain (CTD). Kir2.1 channels are strongly inward-rectifying potassium channels that play a key role in maintaining resting membrane potential. Their gating is modulated by phosphatidylinositol 4,5-bisphosphate (PIP). Genetically inherited defects in Kir2.1 channels are responsible for several rare human diseases, including Andersen's syndrome. The structural analysis (cryo-electron microscopy), surface plasmon resonance, and electrophysiological experiments revealed a well-connected network of interactions between the PIP-binding site and the G-loop through residues R312 and H221. In addition, molecular dynamics simulations and normal mode analysis showed the intrinsic tendency of the CTD to tether to the TMD and a movement of the secondary anionic binding site to the membrane even without PIP. Our results revealed structural features unique to human Kir2.1 and provided insights into the connection between G-loop and gating and the pathological mechanisms associated with this channel.
External linksSci Adv / PubMed:36149965 / PubMed Central
MethodsEM (single particle)
Resolution4.3 Å
Structure data

14678

7zdz

EMDB-14678, PDB-7zdz:
Cryo-EM structure of the human inward-rectifier potassium 2.1 channel (Kir2.1)
Method: EM (single particle) / Resolution: 4.3 Å

Chemicals

ChemComp-K:
Unknown entry

ChemComp-SR:
STRONTIUM ION / Strontium

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / Potassium channel / Inward-rectifier channel / inward rectification

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