EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Transcription factors modulate RNA polymerase conformational equilibrium.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 1546, Year 2022
掲載日	2022年3月22日
著者	Chengjin Zhu / Xieyang Guo / Philippe Dumas / Maria Takacs / Mo'men Abdelkareem / Arnaud Vanden Broeck / Charlotte Saint-André / Gabor Papai / Corinne Crucifix / Julio Ortiz / Albert Weixlbaumer /
PubMed 要旨	RNA polymerase (RNAP) frequently pauses during the transcription of DNA to RNA to regulate gene expression. Transcription factors NusA and NusG modulate pausing, have opposing roles, but can bind ...RNA polymerase (RNAP) frequently pauses during the transcription of DNA to RNA to regulate gene expression. Transcription factors NusA and NusG modulate pausing, have opposing roles, but can bind RNAP simultaneously. Here we report cryo-EM reconstructions of Escherichia coli RNAP bound to NusG, or NusA, or both. RNAP conformational changes, referred to as swivelling, correlate with transcriptional pausing. NusA facilitates RNAP swivelling to further increase pausing, while NusG counteracts this role. Their structural effects are consistent with biochemical results on two categories of transcriptional pauses. In addition, the structures suggest a cooperative mechanism of NusA and NusG during Rho-mediated transcription termination. Our results provide a structural rationale for the stochastic nature of pausing and termination and how NusA and NusG can modulate it.
リンク	Nat Commun / PubMed:35318334 / PubMed Central
手法	EM (単粒子)
解像度	3.8 - 4.5 Å
構造データ	13706 7py0 EMDB-13706, PDB-7py0: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusG (NusG-EC in more-swiveled conformation) 手法: EM (単粒子) / 解像度: 4.5 Å 13707 7py1 EMDB-13707, PDB-7py1: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusG (the consensus NusG-EC) 手法: EM (単粒子) / 解像度: 3.8 Å 13709 7py3 EMDB-13709, PDB-7py3: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA (the consensus NusA-EC) 手法: EM (単粒子) / 解像度: 3.8 Å 13713 7py5 EMDB-13713, PDB-7py5: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA and NusG (the consensus NusA-NusG-EC) 手法: EM (単粒子) / 解像度: 3.9 Å 13714 7py6 EMDB-13714, PDB-7py6: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA and NusG (NusA and NusG elongation complex in less-swiveled conformation) 手法: EM (単粒子) / 解像度: 4.1 Å 13715 7py7 EMDB-13715, PDB-7py7: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA and NusG (NusA and NusG elongation complex in more-swiveled conformation) 手法: EM (単粒子) / 解像度: 4.1 Å 13716 7py8 EMDB-13716, PDB-7py8: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusG (NusG-EC in less-swiveled conformation) 手法: EM (単粒子) / 解像度: 3.8 Å 13717 7pyj EMDB-13717, PDB-7pyj: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA (NusA elongation complex in less-swiveled conformation) 手法: EM (単粒子) / 解像度: 4.2 Å 13718 7pyk EMDB-13718, PDB-7pyk: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA (NusA elongation complex in more-swiveled conformation) 手法: EM (単粒子) / 解像度: 4.1 Å 13745 7q0j EMDB-13745, PDB-7q0j: RNA polymerase elongation complex in more-swiveled conformation 手法: EM (単粒子) / 解像度: 4.3 Å 13746 7q0k EMDB-13746, PDB-7q0k: RNA polymerase elongation complex in less-swiveled conformation 手法: EM (単粒子) / 解像度: 4.0 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry
由来	escherichia coli (大腸菌) escherichia coli ms 115-1 (大腸菌)
キーワード	TRANSCRIPTION (転写 (生物学)) / NusG / transcription elongation / cryo-EM (低温電子顕微鏡法) / NusA / NusA and NusG / RNAP transcription elongation