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TitleA mechanism of origin licensing control through autoinhibition of S. cerevisiae ORC·DNA·Cdc6.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 1059, Year 2022
Publish dateFeb 25, 2022
AuthorsJan Marten Schmidt / Ran Yang / Ashish Kumar / Olivia Hunker / Jan Seebacher / Franziska Bleichert /
PubMed AbstractThe coordinated action of multiple replicative helicase loading factors is needed for the licensing of replication origins prior to DNA replication. Binding of the Origin Recognition Complex (ORC) to ...The coordinated action of multiple replicative helicase loading factors is needed for the licensing of replication origins prior to DNA replication. Binding of the Origin Recognition Complex (ORC) to DNA initiates the ATP-dependent recruitment of Cdc6, Cdt1 and Mcm2-7 loading, but the structural details for timely ATPase site regulation and for how loading can be impeded by inhibitory signals, such as cyclin-dependent kinase phosphorylation, are unknown. Using cryo-electron microscopy, we have determined several structures of S. cerevisiae ORC·DNA·Cdc6 intermediates at 2.5-2.7 Å resolution. These structures reveal distinct ring conformations of the initiator·co-loader assembly and inactive ATPase site configurations for ORC and Cdc6. The Orc6 N-terminal domain laterally engages the ORC·Cdc6 ring in a manner that is incompatible with productive Mcm2-7 docking, while deletion of this Orc6 region alleviates the CDK-mediated inhibition of Mcm7 recruitment. Our findings support a model in which Orc6 promotes the assembly of an autoinhibited ORC·DNA·Cdc6 intermediate to block origin licensing in response to CDK phosphorylation and to avert DNA re-replication.
External linksNat Commun / PubMed:35217664 / PubMed Central
MethodsEM (single particle)
Resolution2.5 - 2.7 Å
Structure data

25924

7tjf

EMDB-25924, PDB-7tjf:
S. cerevisiae ORC bound to 84 bp ARS1 DNA
Method: EM (single particle) / Resolution: 2.6 Å

25925

7tjh

EMDB-25925, PDB-7tjh:
S. cerevisiae ORC bound to 84 bp ARS1 DNA and Cdc6 (state 1) with flexible Orc6 N-terminal domain
Method: EM (single particle) / Resolution: 2.5 Å

25926

7tji

EMDB-25926, PDB-7tji:
S. cerevisiae ORC bound to 84 bp ARS1 DNA and Cdc6 (state 2) with flexible Orc6 N-terminal domain
Method: EM (single particle) / Resolution: 2.7 Å

25927

7tjj

EMDB-25927, PDB-7tjj:
S. cerevisiae ORC bound to 84 bp ARS1 DNA and Cdc6 (state 1) with docked Orc6 N-terminal domain
Method: EM (single particle) / Resolution: 2.7 Å

25928

7tjk

EMDB-25928, PDB-7tjk:
S. cerevisiae ORC bound to 84 bp ARS1 DNA and Cdc6 (state 2) with docked Orc6 N-terminal domain
Method: EM (single particle) / Resolution: 2.7 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER / Water

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsREPLICATION/DNA / initiator / helicase loader / AAA+ ATPase / REPLICATION-DNA complex

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