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TitleStructure of human glycosylphosphatidylinositol transamidase.
Journal, issue, pagesNat Struct Mol Biol, Vol. 29, Issue 3, Page 203-209, Year 2022
Publish dateFeb 14, 2022
AuthorsHongwei Zhang / Jiawei Su / Bin Li / Yiwei Gao / Mengran Liu / Lingli He / Hao Xu / Yanli Dong / Xuejun Cai Zhang / Yan Zhao /
PubMed AbstractGlycosylphosphatidylinositol (GPI) molecules are complex glycophospholipids and serve as membrane anchors for tethering many proteins to the cell surface. Attaching GPI to the protein in the ...Glycosylphosphatidylinositol (GPI) molecules are complex glycophospholipids and serve as membrane anchors for tethering many proteins to the cell surface. Attaching GPI to the protein in the endoplasmic reticulum (ER) is catalyzed by the transmembrane GPI transamidase (GPIT) complex, which is essential for maturation of the GPI-anchored proteins. The GPIT complex is known to be composed of five subunits: PIGK, PIGU, PIGT, PIGS and GPAA1. Here, we determined the structure of the human GPIT complex at a resolution of 3.1 Å using single-particle cryo-EM, elucidating its overall assembly. The PIGK subunit functions as the catalytic component, in which we identified a C206-H164-N58 triad that is critical for the transamination reaction. Transmembrane helices constitute a widely opened cleft, which is located underneath PIGK, serving as a GPI substrate-binding site. The ubiquitin E3 ligase RNF121 is visualized at the back of the complex and probably serves as a quality control factor for the GPIT complex.
External linksNat Struct Mol Biol / PubMed:35165458
MethodsEM (single particle)
Resolution3.1 - 3.8 Å
Structure data

32336

7w72

EMDB-32336, PDB-7w72:
Structure of a human glycosylphosphatidylinositol (GPI) transamidase
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-32452:
Structure of a human glycosylphosphatidylinositol (GPI) transamidase-RNF121 complex
Method: EM (single particle) / Resolution: 3.8 Å

Chemicals

ChemComp-8JY:
[2-[[(2~{R})-2-hexanoyloxy-3-[(~{E})-hex-3-enoxy]propoxy]-oxidanyl-phosphoryl]oxy-3,4,5,6-tetrakis(oxidanyl)phenyl] (2~{E},4~{E})-hepta-2,4-dienoate

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-CA:
Unknown entry

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / glycosylphosphatidylinositol / transamidase

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