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TitleTriggered Reversible Disassembly of an Engineered Protein Nanocage*.
Journal, issue, pagesAngew Chem Int Ed Engl, Vol. 60, Issue 47, Page 25034-25041, Year 2021
Publish dateNov 15, 2021
AuthorsJesse A Jones / Ajitha S Cristie-David / Michael P Andreas / Tobias W Giessen /
PubMed AbstractProtein nanocages play crucial roles in sub-cellular compartmentalization and spatial control in all domains of life and have been used as biomolecular tools for applications in biocatalysis, drug ...Protein nanocages play crucial roles in sub-cellular compartmentalization and spatial control in all domains of life and have been used as biomolecular tools for applications in biocatalysis, drug delivery, and bionanotechnology. The ability to control their assembly state under physiological conditions would further expand their practical utility. To gain such control, we introduced a peptide capable of triggering conformational change at a key structural position in the largest known encapsulin nanocompartment. We report the structure of the resulting engineered nanocage and demonstrate its ability to disassemble and reassemble on demand under physiological conditions. We demonstrate its capacity for in vivo encapsulation of proteins of choice while also demonstrating in vitro cargo loading capabilities. Our results represent a functionally robust addition to the nanocage toolbox and a novel approach for controlling protein nanocage disassembly and reassembly under mild conditions.
External linksAngew Chem Int Ed Engl / PubMed:34532937 / PubMed Central
MethodsEM (single particle)
Resolution3.57 Å
Structure data

23834

7mh2

EMDB-23834, PDB-7mh2:
T4GALA Engineered Protein Nanocage
Method: EM (single particle) / Resolution: 3.57 Å

Source
  • quasibacillus thermotolerans (bacteria)
KeywordsVIRUS LIKE PARTICLE / Encapsulin / Nanocage / Nanocompartment

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