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TitleCryo-EM reveals new species-specific proteins and symmetry elements in the Dot/Icm T4SS.
Journal, issue, pagesElife, Vol. 10, Year 2021
Publish dateSep 14, 2021
AuthorsMichael J Sheedlo / Clarissa L Durie / Jeong Min Chung / Louise Chang / Jacquelyn Roberts / Michele Swanson / Dana Borden Lacy / Melanie D Ohi /
PubMed Abstract is an opportunistic pathogen that causes the potentially fatal pneumonia known as Legionnaires' disease. The pathology associated with infection depends on bacterial delivery of effector proteins ... is an opportunistic pathogen that causes the potentially fatal pneumonia known as Legionnaires' disease. The pathology associated with infection depends on bacterial delivery of effector proteins into the host via the membrane spanning Dot/Icm type IV secretion system (T4SS). We have determined sub-3.0 Å resolution maps of the Dot/Icm T4SS core complex by single particle cryo-EM. The high-resolution structural analysis has allowed us to identify proteins encoded outside the Dot/Icm genetic locus that contribute to the core T4SS structure. We can also now define two distinct areas of symmetry mismatch, one that connects the C18 periplasmic ring (PR) and the C13 outer membrane cap (OMC) and one that connects the C13 OMC with a 16-fold symmetric dome. Unexpectedly, the connection between the PR and OMC is DotH, with five copies sandwiched between the OMC and PR to accommodate the symmetry mismatch. Finally, we observe multiple conformations in the reconstructions that indicate flexibility within the structure.
External linksElife / PubMed:34519271 / PubMed Central
MethodsEM (single particle)
Resolution2.8 - 4.6 Å
Structure data

24004

7muc

EMDB-24004, PDB-7muc:
Legionella pneumophila Dot/Icm T4SS C1 Reconstruction
Method: EM (single particle) / Resolution: 3.8 Å

24005

7mud

EMDB-24005, PDB-7mud:
Legionella pneumophila Dot/Icm T4SS OMC
Method: EM (single particle) / Resolution: 2.8 Å

24006

7mue

EMDB-24006, PDB-7mue:
Legionella pneumophila Dot/Icm T4SS PR
Method: EM (single particle) / Resolution: 2.8 Å

24018

7muq

EMDB-24018, PDB-7muq:
Reconstruction of the Legionella pneumophila Dot/Icm T4SS 3DVA Map 1
Method: EM (single particle) / Resolution: 4.6 Å

24020

7mus

EMDB-24020, PDB-7mus:
Reconstruction of the Legionella pneumophila Dot/Icm T4SS 3DVA Map 2
Method: EM (single particle) / Resolution: 4.6 Å

24023

7muv

EMDB-24023, PDB-7muv:
Reconstruction of the Legionella pneumophila Dot/Icm T4SS 3DVA Map 3
Method: EM (single particle) / Resolution: 4.6 Å

24024

7muw

EMDB-24024, PDB-7muw:
Reconstruction of the Legionella pneumophila Dot/Icm T4SS 3DVA Map 4
Method: EM (single particle) / Resolution: 4.6 Å

24026

7muy

EMDB-24026, PDB-7muy:
Reconstruction of the Legionella pneumophila Dot/Icm T4SS 3DVA Map 5
Method: EM (single particle) / Resolution: 4.6 Å

Source
  • legionella pneumophila (bacteria)
KeywordsTRANSLOCASE / Legionella / Dot/Icm / Secretion / T4SS

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