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Structure paper

TitleStructural basis for genome packaging, retention, and ejection in human cytomegalovirus.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 4538, Year 2021
Publish dateJul 27, 2021
AuthorsZhihai Li / Jingjing Pang / Lili Dong / Xuekui Yu /
PubMed AbstractHow the human cytomegalovirus (HCMV) genome-the largest among human herpesviruses-is packaged, retained, and ejected remains unclear. We present the in situ structures of the symmetry-mismatched ...How the human cytomegalovirus (HCMV) genome-the largest among human herpesviruses-is packaged, retained, and ejected remains unclear. We present the in situ structures of the symmetry-mismatched portal and the capsid vertex-specific components (CVSCs) of HCMV. The 5-fold symmetric 10-helix anchor-uncommon among known portals-contacts the portal-encircling DNA, which is presumed to squeeze the portal as the genome packaging proceeds. We surmise that the 10-helix anchor dampens this action to delay the portal reaching a "head-full" packaging state, thus facilitating the large genome to be packaged. The 6-fold symmetric turret, latched via a coiled coil to a helix from a major capsid protein, supports the portal to retain the packaged genome. CVSCs at the penton vertices-presumed to increase inner capsid pressure-display a low stoichiometry, which would aid genome retention. We also demonstrate that the portal and capsid undergo conformational changes to facilitate genome ejection after viral cell entry.
External linksNat Commun / PubMed:34315863 / PubMed Central
MethodsEM (single particle)
Resolution4.0 - 6.8 Å
Structure data

EMDB-31290:
Human Cytomegalovirus, the reconstruction of C1 portal vertex in the enveloped capsid
Method: EM (single particle) / Resolution: 5.5 Å

EMDB-31291:
Human Cytomegalovirus, the reconstruction of C1 portal vertex in the partially-enveloped capsid
Method: EM (single particle) / Resolution: 4.8 Å

EMDB-31292:
Human Cytomegalovirus, the reconstruction of C1 capsid in the enveloped capsid
Method: EM (single particle) / Resolution: 6.8 Å

EMDB-31293:
Human Cytomegalovirus, the reconstruction of C1 capsid in the partially-enveloped capsid
Method: EM (single particle) / Resolution: 6.3 Å

EMDB-31295:
Human Cytomegalovirus, the reconstruction of C12 portal in the enveloped capsid
Method: EM (single particle) / Resolution: 4.5 Å

31296

7et2

EMDB-31296, PDB-7et2:
Human Cytomegalovirus, C12 portal
Method: EM (single particle) / Resolution: 4.2 Å

31297

7et3

EMDB-31297, PDB-7et3:
C5 portal vertex in the enveloped virion capsid
Method: EM (single particle) / Resolution: 4.2 Å

31298

7etj

EMDB-31298, PDB-7etj:
C5 portal vertex in the partially-enveloped virion capsid
Method: EM (single particle) / Resolution: 4.0 Å

31299

7etm

EMDB-31299, PDB-7etm:
C6 portal vertex in the enveloped virion capsid
Method: EM (single particle) / Resolution: 5.9 Å

EMDB-31300:
Human Cytomegalovirus, C6 reconstruction of the portal vertex in partially-enveloped capsid
Method: EM (single particle) / Resolution: 5.3 Å

31301

7eto

EMDB-31301, PDB-7eto:
C1 CVSC-binding penton vertex in the virion capsid of Human Cytomegalovirus
Method: EM (single particle) / Resolution: 4.0 Å

Source
  • human cytomegalovirus
  • HHV-5, Human herpesvirus 5
KeywordsVIRAL PROTEIN / C12 portal / partially-enveloped capsid / Human Cytomegalovirus / C5 portal vertex / enveloped capsid / C6 portal vertex / C1 CVSC-binding penton vertex / virion capsid

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