EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
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IF	>30 >20 >10 >5 all

タイトル	Structure and immune recognition of the porcine epidemic diarrhea virus spike protein.
ジャーナル・号・ページ	Structure, Vol. 29, Issue 4, Page 385-392.e5, Year 2021
掲載日	2021年4月1日
著者	Robert N Kirchdoerfer / Mahesh Bhandari / Olnita Martini / Leigh M Sewall / Sandhya Bangaru / Kyoung-Jin Yoon / Andrew B Ward /
PubMed 要旨	Porcine epidemic diarrhea virus (PEDV) is an alphacoronavirus responsible for significant morbidity and mortality in pigs. A key determinant of viral tropism and entry, the PEDV spike protein is a ...Porcine epidemic diarrhea virus (PEDV) is an alphacoronavirus responsible for significant morbidity and mortality in pigs. A key determinant of viral tropism and entry, the PEDV spike protein is a key target for the host antibody response and a good candidate for a protein-based vaccine immunogen. We used electron microscopy to evaluate the PEDV spike structure, as well as pig polyclonal antibody responses to viral infection. The structure of the PEDV spike reveals a configuration similar to that of HuCoV-NL63. Several PEDV protein-protein interfaces are mediated by non-protein components, including a glycan at Asn264 and two bound palmitoleic acid molecules. The polyclonal antibody response to PEDV infection shows a dominance of epitopes in the S1 region. This structural and immune characterization provides insights into coronavirus spike stability determinants and explores the immune landscape of viral spike proteins.
リンク	Structure / PubMed:33378641 / PubMed Central
手法	EM (単粒子)
解像度	3.5 - 28.0 Å
構造データ	21391 6vv5 EMDB-21391, PDB-6vv5: Cryo-EM structure of porcine epidemic diarrhea virus (PEDV) spike protein 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-21392: Porcine epidemic diarrhea virus (PEDV) spike protein, expressed in Sf9 cells and negatively stained 手法: EM (単粒子) / 解像度: 13.0 Å EMDB-21393: Porcine epidemic diarrhea virus (PEDV) spike protein, expressed in 293F cells and negatively stained 手法: EM (単粒子) / 解像度: 12.0 Å EMDB-21394: Porcine epidemic diarrhea virus (PEDV) spike protein, expressed in 293F cells with kifunensine and negatively stained 手法: EM (単粒子) / 解像度: 10.0 Å EMDB-21395: Porcine epidemic diarrhea virus (PEDV) spike protein, expressed in 293S cells and negatively stained 手法: EM (単粒子) / 解像度: 19.0 Å EMDB-21396: Porcine epidemic diarrhea virus (PEDV) spike protein with N264D mutation, expressed in 293F cells and negatively stained 手法: EM (単粒子) / 解像度: 18.0 Å EMDB-21397: Porcine epidemic diarrhea virus (PEDV) spike protein, N-terminal domain deletion, expressed in Sf9 cells and negatively stained 手法: EM (単粒子) / 解像度: 23.0 Å EMDB-21398: Porcine epidemic diarrhea virus (PEDV) spike protein bound to negative control polyclonal Fab 手法: EM (単粒子) / 解像度: 15.0 Å EMDB-21399: Porcine epidemic diarrhea virus (PEDV) spike protein bound to polyclonal Fab sample 1, side-binding Fab 手法: EM (単粒子) / 解像度: 22.0 Å EMDB-21400: Porcine epidemic diarrhea virus (PEDV) spike protein bound to polyclonal Fab sample 1, apex-binding Fab 手法: EM (単粒子) / 解像度: 28.0 Å EMDB-21401: Porcine epidemic diarrhea virus (PEDV) spike protein bound to polyclonal Fab sample 2, side-binding Fab 手法: EM (単粒子) / 解像度: 23.0 Å EMDB-21402: Porcine epidemic diarrhea virus (PEDV) spike protein bound to polyclonal Fab sample 2, apex-binding Fab 手法: EM (単粒子) / 解像度: 23.0 Å EMDB-21403: Porcine epidemic diarrhea virus (PEDV) spike protein bound to polyclonal Fab sample 3, side-binding Fab 手法: EM (単粒子) / 解像度: 15.0 Å EMDB-21404: Porcine epidemic diarrhea virus (PEDV) spike protein bound to polyclonal Fab sample 3, apex-binding Fab 手法: EM (単粒子) / 解像度: 17.0 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-PAM: PALMITOLEIC ACID / パルミトレイン酸 / パルミトレイン酸
由来	porcine epidemic diarrhea virus (ブタ流行性下痢ウイルス)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / glycoprotein (糖タンパク質) / surface (表面) / receptor-binding / membrane fusion