EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Allosteric coupling between α-rings of the 20S proteasome.
ジャーナル・号・ページ	Nat Commun, Vol. 11, Issue 1, Page 4580, Year 2020
掲載日	2020年9月11日
著者	Zanlin Yu / Yadong Yu / Feng Wang / Alexander G Myasnikov / Philip Coffino / Yifan Cheng /
PubMed 要旨	Proteasomal machinery performs essential regulated protein degradation in eukaryotes. Classic proteasomes are symmetric, with a regulatory ATPase docked at each end of the cylindrical 20S. Asymmetric ...Proteasomal machinery performs essential regulated protein degradation in eukaryotes. Classic proteasomes are symmetric, with a regulatory ATPase docked at each end of the cylindrical 20S. Asymmetric complexes are also present in cells, either with a single ATPase or with an ATPase and non-ATPase at two opposite ends. The mechanism that populates these different proteasomal complexes is unknown. Using archaea homologs, we construct asymmetric forms of proteasomes. We demonstrate that the gate conformation of the two opposite ends of 20S are coupled: binding one ATPase opens a gate locally, and also opens the opposite gate allosterically. Such allosteric coupling leads to cooperative binding of proteasomal ATPases to 20S and promotes formation of proteasomes symmetrically configured with two identical ATPases. It may also promote formation of asymmetric complexes with an ATPase and a non-ATPase at opposite ends. We propose that in eukaryotes a similar mechanism regulates the composition of the proteasomal population.
リンク	Nat Commun / PubMed:32917864 / PubMed Central
手法	EM (単粒子)
解像度	2.87 - 3.4 Å
構造データ	EMDB-20760: PA26PANc-T20S singly-capped 手法: EM (単粒子) / 解像度: 2.87 Å 20877 6utf EMDB-20877, PDB-6utf: Allosteric coupling between alpha-rings of the 20S proteasome, archaea 20S proteasome singly capped with a PAN complex 手法: EM (単粒子) / 解像度: 3.4 Å 20878 6utg EMDB-20878, PDB-6utg: Allosteric coupling between alpha-rings of the 20S proteasome, 20S singly capped with a PA26/V230F 手法: EM (単粒子) / 解像度: 3.4 Å 20879 6uth EMDB-20879, PDB-6uth: Allosteric coupling between alpha-rings of 20S proteasome, 20S proteasome singly capped with a PA26/E102A_PANc, together with LFP incubation 手法: EM (単粒子) / 解像度: 3.4 Å 20880 6uti EMDB-20880, PDB-6uti: Allosteric coupling between alpha-rings of 20S proteasome, 20S proteasome with singly capped PAN complex 手法: EM (単粒子) / 解像度: 3.4 Å 20881 6utj EMDB-20881: Allosteric couple between alpha rings of the 20S proteasome, 20S proteasome singly capped by PA26/E102A, C-terminus replaced by PAN C-terminus PDB-6utj: Allosteric couple between alpha rings of the 20S proteasome. 20S proteasome singly capped by PA26/E102A, C-terminus replaced by PAN C-terminus 手法: EM (単粒子) / 解像度: 2.9 Å
由来	thermoplasma acidophilum (好酸性) trypanosoma brucei (トリパノソーマ) trypanosoma brucei brucei (ブルーストリパノソーマ)
キーワード	HYDROLASE (加水分解酵素) / proteasome (プロテアソーム) / PAN / singly-capped / PA26/V230F / PA26PANc / substrate LFP / PA26/E102A_PANc