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TitleNetworks of HIV-1 Envelope Glycans Maintain Antibody Epitopes in the Face of Glycan Additions and Deletions.
Journal, issue, pagesStructure, Vol. 28, Issue 8, Page 897-909.e6, Year 2020
Publish dateAug 4, 2020
AuthorsGemma E Seabright / Christopher A Cottrell / Marit J van Gils / Alessio D'addabbo / David J Harvey / Anna-Janina Behrens / Joel D Allen / Yasunori Watanabe / Nicole Scaringi / Thomas M Polveroni / Allison Maker / Snezana Vasiljevic / Natalia de Val / Rogier W Sanders / Andrew B Ward / Max Crispin /
PubMed AbstractNumerous broadly neutralizing antibodies (bnAbs) have been identified that target the glycans of the HIV-1 envelope spike. Neutralization breadth is notable given that glycan processing can be ...Numerous broadly neutralizing antibodies (bnAbs) have been identified that target the glycans of the HIV-1 envelope spike. Neutralization breadth is notable given that glycan processing can be substantially influenced by the presence or absence of neighboring glycans. Here, using a stabilized recombinant envelope trimer, we investigate the degree to which mutations in the glycan network surrounding an epitope impact the fine glycan processing of antibody targets. Using cryo-electron microscopy and site-specific glycan analysis, we reveal the importance of glycans in the formation of the 2G12 bnAb epitope and show that the epitope is only subtly impacted by variations in the glycan network. In contrast, we show that the PG9 and PG16 glycan-based epitopes at the trimer apex are dependent on the presence of the highly conserved surrounding glycans. Glycan networks underpin the conservation of bnAb epitopes and are an important parameter in immunogen design.
External linksStructure / PubMed:32433992 / PubMed Central
MethodsEM (single particle)
Resolution3.79 Å
Structure data

20224

6ozc

EMDB-20224, PDB-6ozc:
BG505 SOSIP.664 with 2G12 Fab2
Method: EM (single particle) / Resolution: 3.79 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

Source
  • human immunodeficiency virus 1
  • homo sapiens (human)
KeywordsVIRAL PROTEIN / HIV Env

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