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TitleStructure-function analysis of silkworm sucrose hydrolase uncovers the mechanism of substrate specificity in GH13 subfamily 17exo-alpha-glucosidases.
Journal, issue, pagesJ. Biol. Chem., Vol. 295, Page 8784-8797, Year 2020
Publish dateDec 5, 2019 (structure data deposition date)
AuthorsMiyazaki, T. / Park, E.Y.
External linksJ. Biol. Chem. / PubMed:32381508
MethodsX-ray diffraction
Resolution1.6 - 1.9 Å
Structure data

PDB-6lga:
Bombyx mori GH13 sucrose hydrolase
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-6lgb:
Bombyx mori GH13 sucrose hydrolase complexed with glucose
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-6lgc:
Bombyx mori GH13 sucrose hydrolase complexed with 1-deoxynojirimycin
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-6lgd:
Bombyx mori GH13 sucrose hydrolase complexed with 1,4-dideoxy-1,4-imino-D-arabinitol
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-6lge:
Bombyx mori GH13 sucrose hydrolase complexed with acarbose
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-6lgf:
Bombyx mori GH13 sucrose hydrolase mutant D247N complexed with sucrose
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-6lgg:
Bombyx mori GH13 sucrose hydrolase mutant E322Q complexed with sucrose
Method: X-RAY DIFFRACTION / Resolution: 1.84 Å

PDB-6lgh:
Bombyx mori GH13 sucrose hydrolase mutant E322Q covalent intermediate
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-6lgi:
Bombyx mori GH13 sucrose hydrolase mutant E322Q covalent intermediate complexed with fructose
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-CA:
Unknown entry

ChemComp-GOL:
GLYCEROL / Glycerol

ChemComp-PGE:
TRIETHYLENE GLYCOL / Polyethylene glycol

ChemComp-HOH:
WATER / Water

ChemComp-GLC:
alpha-D-glucopyranose / Glucose

ChemComp-NOJ:
1-DEOXYNOJIRIMYCIN / inhibitor*YM / 1-Deoxynojirimycin

ChemComp-1AB:
1,4-DIDEOXY-1,4-IMINO-D-ARABINITOL

ChemComp-BGC:
beta-D-glucopyranose / Glucose

ChemComp-FRU:
beta-D-fructofuranose / Fructose

ChemComp-BDF:
beta-D-fructopyranose / Fructose

ChemComp-ACT:
ACETATE ION / Acetate

Source
  • bombyx mori (domestic silkworm)
KeywordsHYDROLASE / Sucrose / Glycoside hydrolase / GH13 / Inhibitor

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