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Structure paper

TitleStructure of an endosomal signaling GPCR-G protein-β-arrestin megacomplex.
Journal, issue, pagesNat Struct Mol Biol, Vol. 26, Issue 12, Page 1123-1131, Year 2019
Publish dateNov 18, 2019
AuthorsAnthony H Nguyen / Alex R B Thomsen / Thomas J Cahill / Rick Huang / Li-Yin Huang / Tara Marcink / Oliver B Clarke / Søren Heissel / Ali Masoudi / Danya Ben-Hail / Fadi Samaan / Venkata P Dandey / Yong Zi Tan / Chuan Hong / Jacob P Mahoney / Sarah Triest / John Little / Xin Chen / Roger Sunahara / Jan Steyaert / Henrik Molina / Zhiheng Yu / Amedee des Georges / Robert J Lefkowitz /
PubMed AbstractClassically, G-protein-coupled receptors (GPCRs) are thought to activate G protein from the plasma membrane and are subsequently desensitized by β-arrestin (β-arr). However, some GPCRs continue to ...Classically, G-protein-coupled receptors (GPCRs) are thought to activate G protein from the plasma membrane and are subsequently desensitized by β-arrestin (β-arr). However, some GPCRs continue to signal through G protein from internalized compartments, mediated by a GPCR-G protein-β-arr 'megaplex'. Nevertheless, the molecular architecture of the megaplex remains unknown. Here, we present its cryo-electron microscopy structure, which shows simultaneous engagement of human G protein and bovine β-arr to the core and phosphorylated tail, respectively, of a single active human chimeric β-adrenergic receptor with the C-terminal tail of the arginine vasopressin type 2 receptor (βVR). All three components adopt their canonical active conformations, suggesting that a single megaplex GPCR is capable of simultaneously activating G protein and β-arr. Our findings provide a structural basis for GPCR-mediated sustained internalized G protein signaling.
External linksNat Struct Mol Biol / PubMed:31740855 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 4.84 Å
Structure data

EMDB-9375, PDB-6ni2:
Stabilized beta-arrestin 1-V2T subcomplex of a GPCR-G protein-beta-arrestin mega-complex
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-9376, PDB-6ni3:
B2V2R-Gs protein subcomplex of a GPCR-G protein-beta-arrestin mega-complex
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-9377:
Structure of a GPCR-G protein-beta-arrestin mega-complex
Method: EM (single particle) / Resolution: 4.84 Å

Chemicals

ChemComp-P0G:
8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one

Source
  • lama glama (llama)
  • bos taurus (cattle)
  • synthetic construct (others)
  • homo sapiens (human)
  • enterobacteria phage rb59 (virus)
KeywordsSIGNALING PROTEIN/IMMUNE SYSTEM / arrestin / GPCR signaling transducer / scaffolding protein / SIGNALING PROTEIN-IMMUNE SYSTEM complex / GPCR / Gs protein / signaling transducer

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