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-Structure paper
タイトル | Structural and Functional Insights into GluK3-kainate Receptor Desensitization and Recovery. |
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ジャーナル・号・ページ | Sci Rep, Vol. 9, Issue 1, Page 10254, Year 2019 |
掲載日 | 2019年7月16日 |
著者 | Jyoti Kumari / Rajesh Vinnakota / Janesh Kumar / |
PubMed 要旨 | GluK3-kainate receptors are atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission. For a better ...GluK3-kainate receptors are atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission. For a better understanding of structural changes that underlie receptor functions, GluK3 receptors were trapped in desensitized and resting/closed states and structures analyzed using single particle cryo-electron microscopy. While the desensitized GluK3 has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound GluK3 trapped a resting state with only two LBD domains in dimeric arrangement necessary for receptor activation. Using structures as a guide, we show that the N-linked glycans at the interface of GluK3 ATD and LBD likely mediate inter-domain interactions and attune receptor-gating properties. The mutational analysis also identified putative N-glycan interacting residues. Our results provide a molecular framework for understanding gating properties unique to GluK3 and exploring the role of N-linked glycosylation in their modulation. |
リンク | Sci Rep / PubMed:31311973 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 1.832 - 7.6 Å |
構造データ | PDB-6jmv: |
化合物 | ChemComp-SYM: ChemComp-CL: ChemComp-ZN: ChemComp-GOL: ChemComp-ACT: ChemComp-NA: ChemComp-HOH: |
由来 |
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キーワード | MEMBRANE PROTEIN (膜タンパク質) / Glutamate receptor (グルタミン酸受容体) / Kainate (カイニン酸) / SYM / UBP310 |