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TitleStructure and activity of lipid bilayer within a membrane-protein transporter.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 115, Issue 51, Page 12985-12990, Year 2018
Publish dateDec 18, 2018
AuthorsWeihua Qiu / Ziao Fu / Guoyan G Xu / Robert A Grassucci / Yan Zhang / Joachim Frank / Wayne A Hendrickson / Youzhong Guo /
PubMed AbstractMembrane proteins function in native cell membranes, but extraction into isolated particles is needed for many biochemical and structural analyses. Commonly used detergent-extraction methods destroy ...Membrane proteins function in native cell membranes, but extraction into isolated particles is needed for many biochemical and structural analyses. Commonly used detergent-extraction methods destroy naturally associated lipid bilayers. Here, we devised a detergent-free method for preparing cell-membrane nanoparticles to study the multidrug exporter AcrB, by cryo-EM at 3.2-Å resolution. We discovered a remarkably well-organized lipid-bilayer structure associated with transmembrane domains of the AcrB trimer. This bilayer patch comprises 24 lipid molecules; inner leaflet chains are packed in a hexagonal array, whereas the outer leaflet has highly irregular but ordered packing. Protein side chains interact with both leaflets and participate in the hexagonal pattern. We suggest that the lipid bilayer supports and harmonizes peristaltic motions through AcrB trimers. In AcrB D407A, a putative proton-relay mutant, lipid bilayer buttresses protein interactions lost in crystal structures after detergent-solubilization. Our detergent-free system preserves lipid-protein interactions for visualization and should be broadly applicable.
External linksProc Natl Acad Sci U S A / PubMed:30509977 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 3.2 Å
Structure data

7074

6baj

EMDB-7074: AcrB
PDB-6baj: Cryo-EM structure of lipid bilayer in the native cell membrane nanoparticles of AcrB
Method: EM (single particle) / Resolution: 3.2 Å

7609

6csx

EMDB-7609: AcrBD407 mutant
PDB-6csx: Single particles Cryo-EM structure of AcrB D407A associated with lipid bilayer at 3.0 Angstrom
Method: EM (single particle) / Resolution: 3.0 Å

Chemicals

ChemComp-PTY:
PHOSPHATIDYLETHANOLAMINE / phospholipid*YM / Phosphatidylethanolamine

ChemComp-D12:
DODECANE / Dodecane

ChemComp-HOH:
WATER / Water

Source
  • Escherichia coli (E. coli)
  • escherichia coli (strain k12) (bacteria)
KeywordsMEMBRANE PROTEIN / lipid bilayer / native cell membrane nanoparticles system / Resistance-Nodulation-Cell Division (RND) family / phospholipid / TRANSPORT PROTEIN / AcrB / Native cell membrane nanoparticles / SMA

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