EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	emClarity: software for high-resolution cryo-electron tomography and subtomogram averaging.
ジャーナル・号・ページ	Nat Methods, Vol. 15, Issue 11, Page 955-961, Year 2018
掲載日	2018年10月22日
著者	Benjamin A Himes / Peijun Zhang /
PubMed 要旨	Macromolecular complexes are intrinsically flexible and often challenging to purify for structure determination by single-particle cryo-electron microscopy (cryo-EM). Such complexes can be studied by ...Macromolecular complexes are intrinsically flexible and often challenging to purify for structure determination by single-particle cryo-electron microscopy (cryo-EM). Such complexes can be studied by cryo-electron tomography (cryo-ET) combined with subtomogram alignment and classification, which in exceptional cases achieves subnanometer resolution, yielding insight into structure-function relationships. However, it remains challenging to apply this approach to specimens that exhibit conformational or compositional heterogeneity or are present in low abundance. To address this, we developed emClarity ( https://github.com/bHimes/emClarity/wiki ), a GPU-accelerated image-processing package featuring an iterative tomographic tilt-series refinement algorithm that uses subtomograms as fiducial markers and a 3D-sampling-function-compensated, multi-scale principal component analysis classification method. We demonstrate that our approach offers substantial improvement in the resolution of maps and in the separation of different functional states of macromolecular complexes compared with current state-of-the-art software.
リンク	Nat Methods / PubMed:30349041 / PubMed Central
手法	EM (サブトモグラム平均)
解像度	3.1 - 11.0 Å
構造データ	EMDB-8799: Yeast 80S ribosome derived from EMPIAR-10045 using emClarity 手法: EM (サブトモグラム平均) / 解像度: 7.8 Å EMDB-8802: 80S ribsome from Oryctolagus cuniculus, class I - with non-rotated/non-swiveled 40S and P site tRNA, derived from EMPIAR-10064 using eClarity 手法: EM (サブトモグラム平均) / 解像度: 9.3 Å EMDB-8803: 80S ribsome from Oryctolagus cuniculus, class II - with non-rotated/non-swiveled 40S with P site tRNA and ternary complex, derived from EMPIAR-10064 using eClarity 手法: EM (サブトモグラム平均) / 解像度: 9.9 Å EMDB-8804: 80S ribsome from Oryctolagus cuniculus, class III - with non-rotated/non-swiveled 40S with P site and E site tRNA, derived from EMPIAR-10064 using eClarity 手法: EM (サブトモグラム平均) / 解像度: 10.2 Å EMDB-8805: 80S ribsome from Oryctolagus cuniculus, class IV - with mid-rotated/swiveled 40S and eeF2, derived from EMPIAR-10064 using eClarity 手法: EM (サブトモグラム平均) / 解像度: 9.8 Å EMDB-8806: 80S ribsome from Oryctolagus cuniculus, class V - with mid-rotated/swiveled 40S and eeF2, derived from EMPIAR-10064 using eClarity 手法: EM (サブトモグラム平均) / 解像度: 11.0 Å EMDB-8986: A 3.1 Angstrom sub-tomogram average of HIV-1 CA-SP1. 手法: EM (サブトモグラム平均) / 解像度: 3.1 Å
由来	Saccharomyces cerevisiae (パン酵母) Oryctolagus cuniculus (ウサギ)