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TitleArchitectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering.
Journal, issue, pagesStructure, Vol. 26, Issue 8, Page 1127-11136.e4, Year 2018
Publish dateAug 7, 2018
AuthorsKai Cai / Ronnie O Frederick / Hesam Dashti / John L Markley /
PubMed AbstractCysteine desulfurase plays a central role in mitochondrial iron-sulfur cluster biogenesis by generating sulfur through the conversion of L-cysteine to L-alanine and by serving as the platform for ...Cysteine desulfurase plays a central role in mitochondrial iron-sulfur cluster biogenesis by generating sulfur through the conversion of L-cysteine to L-alanine and by serving as the platform for assembling other components of the biosynthetic machinery, including ISCU, frataxin, and ferredoxin. The human mitochondrial cysteine desulfurase complex consists of two copies each of NFS1, ISD11, and acyl carrier protein. We describe results from chemical crosslinking coupled with tandem mass spectrometry and small-angle X-ray scattering studies that are consistent with a closed NFS1 dimer rather than an open one for both the cysteine desulfurase-ISCU and cysteine desulfurase-ISCU-frataxin complexes. We present a structural model for the cysteine desulfurase-ISCU-frataxin complex derived from chemical crosslinking restraints in conjunction with the recent crystal structure of the cysteine desulfurase-ISCU-zinc complex and distance constraints from nuclear magnetic resonance.
External linksStructure / PubMed:29983374 / PubMed Central
MethodsSAS (X-ray in house)
Structure data

SASDDB3:
Human mitochondrial cysteine desulfurase (NFS1, ISD11 and Acp heterodimer complex)
Method: SAXS/SANS

SASDDC3:
Human mitochondrial cysteine desulfurase-ISCU (NFS1, ISD11, Acp and ISCU heterodimer complex)
Method: SAXS/SANS

SASDDD3:
Human mitochondrial cysteine desulfurase-ISCU-Frataxin (NFS1, ISD11 and Acp heterodimer complex)
Method: SAXS/SANS

Source
  • Homo sapiens (human)
  • Escherichia coli (strain k12) (bacteria)

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