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| Title | Hexameric and pentameric complexes of the ExbBD energizer in the Ton system. |
|---|---|
| Journal, issue, pages | Elife, Vol. 7, Year 2018 |
| Publish date | Apr 17, 2018 |
 Authors | Saori Maki-Yonekura / Rei Matsuoka / Yoshiki Yamashita / Hirofumi Shimizu / Maiko Tanaka / Fumie Iwabuki / Koji Yonekura /  |
| PubMed Abstract | Gram-negative bacteria import essential nutrients such as iron and vitamin B through outer membrane receptors. This process utilizes proton motive force harvested by the Ton system made up of three ...Gram-negative bacteria import essential nutrients such as iron and vitamin B through outer membrane receptors. This process utilizes proton motive force harvested by the Ton system made up of three inner membrane proteins, ExbB, ExbD and TonB. ExbB and ExbD form the proton channel that energizes uptake through TonB. Recently, crystal structures suggest that the ExbB pentamer is the scaffold. Here, we present structures of hexameric complexes of ExbB and ExbD revealed by X-ray crystallography and single particle cryo-EM. Image analysis shows that hexameric and pentameric complexes coexist, with the proportion of hexamer increasing with pH. Channel current measurement and 2D crystallography support the existence and transition of the two oligomeric states in membranes. The hexameric complex consists of six ExbB subunits and three ExbD transmembrane helices enclosed within the central channel. We propose models for activation/inactivation associated with hexamer and pentamer formation and utilization of proton motive force. |
 External links | Elife / PubMed:29661272 / PubMed Central |
| Methods | EM (single particle) / X-ray diffraction |
| Resolution | 2.84 - 7.1 Å |
| Structure data | EMDB-6927, PDB-5zfu: EMDB-6928, PDB-5zfv:  PDB-5zfp: |
| Chemicals |  ChemComp-HOH: |
| Source |
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 Keywords |  TRANSPORT PROTEIN / TON SYSTEM / ION CHANNEL / ENERGIZER / TRANSPORTER / PROTON MOTIVE FORCE |
