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TitleAtomic Resolution Structures of Human Bufaviruses Determined by Cryo-Electron Microscopy.
Journal, issue, pagesViruses, Vol. 10, Issue 1, Year 2018
Publish dateJan 4, 2018
AuthorsMaria Ilyas / Mario Mietzsch / Shweta Kailasan / Elina Väisänen / Mengxiao Luo / Paul Chipman / J Kennon Smith / Justin Kurian / Duncan Sousa / Robert McKenna / Maria Söderlund-Venermo / Mavis Agbandje-McKenna /
PubMed AbstractBufavirus strain 1 (BuV1), a member of the genus of the , was first isolated from fecal samples of children with acute diarrhea in Burkina Faso. Since this initial discovery, BuVs have been isolated ...Bufavirus strain 1 (BuV1), a member of the genus of the , was first isolated from fecal samples of children with acute diarrhea in Burkina Faso. Since this initial discovery, BuVs have been isolated in several countries, including Finland, the Netherlands, and Bhutan, in pediatric patients exhibiting similar symptoms. Towards their characterization, the structures of virus-like particles of BuV1, BuV2, and BuV3, the current known genotypes, have been determined by cryo-electron microscopy and image reconstruction to 2.84, 3.79, and 3.25 Å, respectively. The BuVs, 65-73% identical in amino acid sequence, conserve the major viral protein, VP2, structure and general capsid surface features of parvoviruses. These include a core β-barrel (βB-βI), α-helix A, and large surface loops inserted between these elements in VP2. The capsid contains depressions at the icosahedral 2-fold and around the 5-fold axes, and has three separated protrusions surrounding the 3-fold axes. Structure comparison among the BuVs and to available parvovirus structures revealed capsid surface variations and capsid 3-fold protrusions that depart from the single pinwheel arrangement of the animal protoparvoviruses. These structures provide a platform to begin the molecular characterization of these potentially pathogenic viruses.
External linksViruses / PubMed:29300333 / PubMed Central
MethodsEM (single particle)
Resolution2.84 - 3.79 Å
Structure data

EMDB-7300, PDB-6bwx:
Atomic resolution structure of human bufavirus 1
Method: EM (single particle) / Resolution: 2.84 Å

EMDB-7301, PDB-6bx0:
Atomic resolution structure of human bufavirus 2
Method: EM (single particle) / Resolution: 3.79 Å

EMDB-7302, PDB-6bx1:
Atomic resolution structure of human bufavirus 3
Method: EM (single particle) / Resolution: 3.25 Å

Source
  • bufavirus-1
  • bufavirus-2
  • bufavirus-3
KeywordsVIRUS LIKE PARTICLE / parvovirus / protoparvovirus / Bufavirus

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