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TitleCryo-EM study of start codon selection during archaeal translation initiation.
Journal, issue, pagesNat Commun, Vol. 7, Page 13366, Year 2016
Publish dateNov 7, 2016
AuthorsPierre-Damien Coureux / Christine Lazennec-Schurdevin / Auriane Monestier / Eric Larquet / Lionel Cladière / Bruno P Klaholz / Emmanuelle Schmitt / Yves Mechulam /
PubMed AbstractEukaryotic and archaeal translation initiation complexes have a common structural core comprising e/aIF1, e/aIF1A, the ternary complex (TC, e/aIF2-GTP-Met-tRNA) and mRNA bound to the small ribosomal ...Eukaryotic and archaeal translation initiation complexes have a common structural core comprising e/aIF1, e/aIF1A, the ternary complex (TC, e/aIF2-GTP-Met-tRNA) and mRNA bound to the small ribosomal subunit. e/aIF2 plays a crucial role in this process but how this factor controls start codon selection remains unclear. Here, we present cryo-EM structures of the full archaeal 30S initiation complex showing two conformational states of the TC. In the first state, the TC is bound to the ribosome in a relaxed conformation with the tRNA oriented out of the P site. In the second state, the tRNA is accommodated within the peptidyl (P) site and the TC becomes constrained. This constraint is compensated by codon/anticodon base pairing, whereas in the absence of a start codon, aIF2 contributes to swing out the tRNA. This spring force concept highlights a mechanism of codon/anticodon probing by the initiator tRNA directly assisted by aIF2.
External linksNat Commun / PubMed:27819266 / PubMed Central
MethodsEM (single particle)
Resolution5.34 Å
Structure data

8148

5jb3

EMDB-8148, PDB-5jb3:
Cryo-EM structure of a full archaeal ribosomal translation initiation complex in the P-REMOTE conformation
Method: EM (single particle) / Resolution: 5.34 Å

8149

5jbh

EMDB-8149, PDB-5jbh:
Cryo-EM structure of a full archaeal ribosomal translation initiation complex in the P-IN conformation
Method: EM (single particle) / Resolution: 5.34 Å

Chemicals

ChemComp-MET:
METHIONINE / Methionine

ChemComp-MG:
Unknown entry

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM / 5'-Guanylyl imidodiphosphate

ChemComp-ZN:
Unknown entry

Source
  • pyrococcus abyssi ge5 (archaea)
  • escherichia coli (E. coli)
  • pyrococcus (archaea)
KeywordsTRANSLATION / TRANSCRIPTION

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