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TitleKey Intermediates in Ribosome Recycling Visualized by Time-Resolved Cryoelectron Microscopy.
Journal, issue, pagesStructure, Vol. 24, Issue 12, Page 2092-2101, Year 2016
Publish dateDec 6, 2016
AuthorsZiao Fu / Sandip Kaledhonkar / Anneli Borg / Ming Sun / Bo Chen / Robert A Grassucci / Måns Ehrenberg / Joachim Frank /
PubMed AbstractUpon encountering a stop codon on mRNA, polypeptide synthesis on the ribosome is terminated by release factors, and the ribosome complex, still bound with mRNA and P-site-bound tRNA (post-termination ...Upon encountering a stop codon on mRNA, polypeptide synthesis on the ribosome is terminated by release factors, and the ribosome complex, still bound with mRNA and P-site-bound tRNA (post-termination complex, PostTC), is split into ribosomal subunits, ready for a new round of translational initiation. Separation of post-termination ribosomes into subunits, or "ribosome recycling," is promoted by the joint action of ribosome-recycling factor (RRF) and elongation factor G (EF-G) in a guanosine triphosphate (GTP) hydrolysis-dependent manner. Here we used a mixing-spraying-based method of time-resolved cryo-electron microscopy (cryo-EM) to visualize the short-lived intermediates of the recycling process. The two complexes that contain (1) both RRF and EF-G bound to the PostTC or (2) deacylated tRNA bound to the 30S subunit are of particular interest. Our observations of the native form of these complexes demonstrate the strong potential of time-resolved cryo-EM for visualizing previously unobservable transient structures.
External linksStructure / PubMed:27818103 / PubMed Central
MethodsEM (single particle)
Resolution7.4 - 16.0 Å
Structure data

EMDB-8411:
Time-resolved cryo electron microscopy map of the RRF-bound post-termination ribosome complex
Method: EM (single particle) / Resolution: 10.0 Å

EMDB-8412:
Time-resolved cryo electron microscopy map of the RRF-bound post-termination ribosome complex
Method: EM (single particle) / Resolution: 16.0 Å

EMDB-8413:
Time-resolved cryo electron microscopy map of the EF-G and RRF-bound post-termination ribosome complex
Method: EM (single particle) / Resolution: 7.4 Å

EMDB-8415:
Time-resolved cryo electron microscopy map of the EF-G and RRF-bound 50S subunit
Method: EM (single particle) / Resolution: 12.0 Å

EMDB-8416:
Time-resolved cryo electron microscopy map of the EF-G-, RRF-, and tRNA-bound 50S subunit
Method: EM (single particle) / Resolution: 16.0 Å

EMDB-8417:
Time-resolved cryo electron microscopy map of the tRNA-bound 30S subunit
Method: EM (single particle) / Resolution: 10.0 Å

EMDB-8418:
Time-resolved cryo electron microscopy map of the IF3-bound 30S subunit
Method: EM (single particle) / Resolution: 10.0 Å

Source
  • Escherichia coli (E. coli)

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