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TitleNear-atomic cryo-EM structure of PRC1 bound to the microtubule.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 113, Issue 34, Page 9430-9439, Year 2016
Publish dateAug 23, 2016
AuthorsElizabeth H Kellogg / Stuart Howes / Shih-Chieh Ti / Erney Ramírez-Aportela / Tarun M Kapoor / Pablo Chacón / Eva Nogales /
PubMed AbstractProteins that associate with microtubules (MTs) are crucial to generate MT arrays and establish different cellular architectures. One example is PRC1 (protein regulator of cytokinesis 1), which cross- ...Proteins that associate with microtubules (MTs) are crucial to generate MT arrays and establish different cellular architectures. One example is PRC1 (protein regulator of cytokinesis 1), which cross-links antiparallel MTs and is essential for the completion of mitosis and cytokinesis. Here we describe a 4-Å-resolution cryo-EM structure of monomeric PRC1 bound to MTs. Residues in the spectrin domain of PRC1 contacting the MT are highly conserved and interact with the same pocket recognized by kinesin. We additionally found that PRC1 promotes MT assembly even in the presence of the MT stabilizer taxol. Interestingly, the angle of the spectrin domain on the MT surface corresponds to the previously observed cross-bridge angle between MTs cross-linked by full-length, dimeric PRC1. This finding, together with molecular dynamic simulations describing the intrinsic flexibility of PRC1, suggests that the MT-spectrin domain interface determines the geometry of the MT arrays cross-linked by PRC1.
External linksProc Natl Acad Sci U S A / PubMed:27493215 / PubMed Central
MethodsEM (helical sym.)
Resolution3.5 Å
Structure data

8266

5kmg

EMDB-8266, PDB-5kmg:
Near-atomic cryo-EM structure of PRC1 bound to the microtubule
Method: EM (helical sym.) / Resolution: 3.5 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-POU:
Peloruside A

Source
  • sus scrofa (pig)
  • homo sapiens (human)
KeywordsSTRUCTURAL PROTEIN / cytoskeleton / mitosis / microtubule / microtubule-associated protein

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