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TitleStructure of a yeast catalytic step I spliceosome at 3.4 Å resolution.
Journal, issue, pagesScience, Vol. 353, Issue 6302, Page 895-904, Year 2016
Publish dateAug 26, 2016
AuthorsRuixue Wan / Chuangye Yan / Rui Bai / Gaoxingyu Huang / Yigong Shi /
PubMed AbstractEach cycle of pre-messenger RNA splicing, carried out by the spliceosome, comprises two sequential transesterification reactions, which result in the removal of an intron and the joining of two exons. ...Each cycle of pre-messenger RNA splicing, carried out by the spliceosome, comprises two sequential transesterification reactions, which result in the removal of an intron and the joining of two exons. Here we report an atomic structure of a catalytic step I spliceosome (known as the C complex) from Saccharomyces cerevisiae, as determined by cryo-electron microscopy at an average resolution of 3.4 angstroms. In the structure, the 2'-OH of the invariant adenine nucleotide in the branch point sequence (BPS) is covalently joined to the phosphate at the 5' end of the 5' splice site (5'SS), forming an intron lariat. The freed 5' exon remains anchored to loop I of U5 small nuclear RNA (snRNA), and the 5'SS and BPS of the intron form duplexes with conserved U6 and U2 snRNA sequences, respectively. Specific placement of these RNA elements at the catalytic cavity of Prp8 is stabilized by 15 protein components, including Snu114 and the splicing factors Cwc21, Cwc22, Cwc25, and Yju2. These features, representing the conformation of the spliceosome after the first-step reaction, predict structural changes that are needed for the execution of the second-step transesterification reaction.
External linksScience / PubMed:27445308
MethodsEM (single particle)
Resolution3.4 - 4.6 Å
Structure data

9525

5gmk

EMDB-9525, PDB-5gmk:
Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-9526:
Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.65 angstrom resolution
Method: EM (single particle) / Resolution: 3.65 Å

EMDB-9527:
Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 4.6 angstrom resolution
Method: EM (single particle) / Resolution: 4.6 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • saccharomyces cerevisiae s288c (yeast)
KeywordsRNA BINDING PROTEIN/RNA / RNA splicing / Spliceosome / Catalytic Step I / Intron lariat / RNA BINDING PROTEIN-RNA complex

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