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TitleStructure of the GTP Form of Elongation Factor 4 (EF4) Bound to the Ribosome.
Journal, issue, pagesJ Biol Chem, Vol. 291, Issue 25, Page 12943-12950, Year 2016
Publish dateJun 17, 2016
AuthorsVeerendra Kumar / Rya Ero / Tofayel Ahmed / Kwok Jian Goh / Yin Zhan / Shashi Bhushan / Yong-Gui Gao /
PubMed AbstractElongation factor 4 (EF4) is a member of the family of ribosome-dependent translational GTPase factors, along with elongation factor G and BPI-inducible protein A. Although EF4 is highly conserved in ...Elongation factor 4 (EF4) is a member of the family of ribosome-dependent translational GTPase factors, along with elongation factor G and BPI-inducible protein A. Although EF4 is highly conserved in bacterial, mitochondrial, and chloroplast genomes, its exact biological function remains controversial. Here we present the cryo-EM reconstitution of the GTP form of EF4 bound to the ribosome with P and E site tRNAs at 3.8-Å resolution. Interestingly, our structure reveals an unrotated ribosome rather than a clockwise-rotated ribosome, as observed in the presence of EF4-GDP and P site tRNA. In addition, we also observed a counterclockwise-rotated form of the above complex at 5.7-Å resolution. Taken together, our results shed light on the interactions formed between EF4, the ribosome, and the P site tRNA and illuminate the GTPase activation mechanism at previously unresolved detail.
External linksJ Biol Chem / PubMed:27137929 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 5.9 Å
Structure data

6584

5imq

EMDB-6584: The structure of elongation factor 4 (EF4/LepA) in GTP form bound to the ribosome
PDB-5imq: Structure of ribosome bound to cofactor at 3.8 angstrom resolution
Method: EM (single particle) / Resolution: 3.8 Å

6585

5imr

EMDB-6585: The structure of elongation factor 4 (EF4/LepA) in GTP form bound to the ribosome
PDB-5imr: Structure of ribosome bound to cofactor at 5.7 angstrom resolution
Method: EM (single particle) / Resolution: 5.9 Å

Chemicals

ChemComp-GCP:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-PCP, energy-carrying molecule analogue*YM

Source
  • Thermus thermophilus (bacteria)
  • thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (bacteria)
  • salmonella enterica subsp. enterica serovar enteritidis str. sa20094389 (bacteria)
KeywordsRIBOSOME / EF4/LepA / translational GTPase factors / translocation / reverse

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