Yi-Wei Chang / Lee A Rettberg / Anke Treuner-Lange / Janet Iwasa / Lotte Søgaard-Andersen / Grant J Jensen /
PubMed Abstract
Type IVa pili are filamentous cell surface structures observed in many bacteria. They pull cells forward by extending, adhering to surfaces, and then retracting. We used cryo-electron tomography of ...Type IVa pili are filamentous cell surface structures observed in many bacteria. They pull cells forward by extending, adhering to surfaces, and then retracting. We used cryo-electron tomography of intact Myxococcus xanthus cells to visualize type IVa pili and the protein machine that assembles and retracts them (the type IVa pilus machine, or T4PM) in situ, in both the piliated and nonpiliated states, at a resolution of 3 to 4 nanometers. We found that T4PM comprises an outer membrane pore, four interconnected ring structures in the periplasm and cytoplasm, a cytoplasmic disc and dome, and a periplasmic stem. By systematically imaging mutants lacking defined T4PM proteins or with individual proteins fused to tags, we mapped the locations of all 10 T4PM core components and the minor pilins, thereby providing insights into pilus assembly, structure, and function.
EMDB-3247: Subtomogram average of a piliated type IVa pilus machine in wild-type Myxococcus xanthus cells PDB-3jc8: Architectural model of the type IVa pilus machine in a piliated state Method: EM (subtomogram averaging)
EMDB-3248: Subtomogram average of a non-piliated type IVa pilus machine in wild-type Myxococcus xanthus cells Method: EM (subtomogram averaging)
EMDB-3249: Subtomogram average of a non-piliated type IVa pilus machine in Myxococcus xanthus cells with pilP knockout Method: EM (subtomogram averaging)
EMDB-3250: Subtomogram average of a piliated type IVa pilus machine in Myxococcus xanthus cells with PilP tagged with a superfolder GFP Method: EM (subtomogram averaging)
EMDB-3251: Subtomogram average of a non-piliated type IVa pilus machine in Myxococcus xanthus cells with PilP tagged with a superfolder GFP Method: EM (subtomogram averaging)
EMDB-3252: Subtomogram average of a piliated type IVa pilus machine in Myxococcus xanthus cells with tsaP knockout Method: EM (subtomogram averaging)
EMDB-3253: Subtomogram average of a non-piliated type IVa pilus machine in Myxococcus xanthus cells with tsaP knockout Method: EM (subtomogram averaging)
EMDB-3254: Subtomogram average of a piliated type IVa pilus machine in Myxococcus xanthus cells with PilO tagged with a superfolder GFP Method: EM (subtomogram averaging)
EMDB-3255: Subtomogram average of a non-piliated type IVa pilus machine in Myxococcus xanthus cells with PilO tagged with a superfolder GFP Method: EM (subtomogram averaging)
EMDB-3256: Subtomogram average of a non-piliated type IVa pilus machine in Myxococcus xanthus cells with pilC knockout Method: EM (subtomogram averaging)
EMDB-3257: Subtomogram average of a non-piliated type IVa pilus machine in Myxococcus xanthus cells with pilA knockout Method: EM (subtomogram averaging)
EMDB-3258: Subtomogram average of a non-piliated type IVa pilus machine in Myxococcus xanthus cells with pilV/pilW/fimU knockout Method: EM (subtomogram averaging)
EMDB-3259: Subtomogram average of a piliated type IVa pilus machine in Myxococcus xanthus cells with pilT knockout Method: EM (subtomogram averaging)
EMDB-3260: Subtomogram average of a non-piliated type IVa pilus machine in Myxococcus xanthus cells with pilB knockout PDB-3jc9: Architectural model of the type IVa pilus machine in a non-piliated state Method: EM (subtomogram averaging)
EMDB-3261: Subtomogram average of a non-piliated type IVa pilus machine in Myxococcus xanthus cells with pilB/pilT knockout Method: EM (subtomogram averaging)
EMDB-3262: Subtomogram average of a non-piliated type IVa pilus machine in Myxococcus xanthus cells with pilM knockout Method: EM (subtomogram averaging)
EMDB-3263: Subtomogram average of a piliated type IVa pilus machine in Myxococcus xanthus cells with pilQ 25-300 residues truncated Method: EM (subtomogram averaging)
EMDB-3264: Subtomogram average of a non-piliated type IVa pilus machine in Myxococcus xanthus cells with pilQ 25-300 residues truncated Method: EM (subtomogram averaging)
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