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TitleStructure of the native Sec61 protein-conducting channel.
Journal, issue, pagesNat Commun, Vol. 6, Page 8403, Year 2015
Publish dateSep 28, 2015
AuthorsStefan Pfeffer / Laura Burbaum / Pia Unverdorben / Markus Pech / Yuxiang Chen / Richard Zimmermann / Roland Beckmann / Friedrich Förster /
PubMed AbstractIn mammalian cells, secretory and membrane proteins are translocated across or inserted into the endoplasmic reticulum (ER) membrane by the universally conserved protein-conducting channel Sec61, ...In mammalian cells, secretory and membrane proteins are translocated across or inserted into the endoplasmic reticulum (ER) membrane by the universally conserved protein-conducting channel Sec61, which has been structurally studied in isolated, detergent-solubilized states. Here we structurally and functionally characterize native, non-solubilized ribosome-Sec61 complexes on rough ER vesicles using cryo-electron tomography and ribosome profiling. Surprisingly, the 9-Å resolution subtomogram average reveals Sec61 in a laterally open conformation, even though the channel is not in the process of inserting membrane proteins into the lipid bilayer. In contrast to recent mechanistic models for polypeptide translocation and insertion, our results indicate that the laterally open conformation of Sec61 is the only conformation present in the ribosome-bound translocon complex, independent of its functional state. Consistent with earlier functional studies, our structure suggests that the ribosome alone, even without a nascent chain, is sufficient for lateral opening of Sec61 in a lipid environment.
External linksNat Commun / PubMed:26411746 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution9.0 - 10.0 Å
Structure data

3068

5a6u

EMDB-3068: Mammalian ribosome bound to the native Sec61 protein-conducting channel in the 'non-inserting' state ('conventional' alignment)
PDB-5a6u: Native mammalian ribosome-bound Sec61 protein-conducting channel in the 'non-inserting' state
Method: EM (subtomogram averaging) / Resolution: 9.0 Å

EMDB-3069:
Mammalian ribosome bound to the native Sec61 protein-conducting channel in the 'non-inserting' state ('gold standard' alignment)
Method: EM (subtomogram averaging) / Resolution: 9.0 Å

EMDB-3070:
Mammalian ribosome bound to the native Sec61 protein-conducting channel in the idle state
Method: EM (subtomogram averaging) / Resolution: 9.4 Å

EMDB-3071:
Mammalian ribosome bound to the native Sec61 protein-conducting channel in the translocating state
Method: EM (subtomogram averaging) / Resolution: 10.0 Å

EMDB-3072:
Representative tomogram of canine pancreatic rough Endoplasmic Reticulum vesicles
Method: EM (tomography)

Source
  • canis lupus familiaris (dog)
KeywordsTRANSLATION / RIBOSOME / SEC61 / TRANSLOCON / ENDOPLASMIC RETICULUM / CRYOELECTRON TOMOGRAPHY / SUBTOMOGRAM ANALYSIS

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