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TitleStructure and gating of the nuclear pore complex.
Journal, issue, pagesNat Commun, Vol. 6, Page 7532, Year 2015
Publish dateJun 26, 2015
AuthorsMatthias Eibauer / Mauro Pellanda / Yagmur Turgay / Anna Dubrovsky / Annik Wild / Ohad Medalia /
PubMed AbstractNuclear pore complexes (NPCs) perforate the nuclear envelope and allow the exchange of macromolecules between the nucleus and the cytoplasm. To acquire a deeper understanding of this transport ...Nuclear pore complexes (NPCs) perforate the nuclear envelope and allow the exchange of macromolecules between the nucleus and the cytoplasm. To acquire a deeper understanding of this transport mechanism, we analyse the structure of the NPC scaffold and permeability barrier, by reconstructing the Xenopus laevis oocyte NPC from native nuclear envelopes up to 20 Å resolution by cryo-electron tomography in conjunction with subtomogram averaging. In addition to resolving individual protein domains of the NPC constituents, we propose a model for the architecture of the molecular gate at its central channel. Furthermore, we compare and contrast this native NPC structure to one that exhibits reduced transport activity and unveil the spatial properties of the NPC gate.
External linksNat Commun / PubMed:26112706 / PubMed Central
MethodsEM (subtomogram averaging)
Structure data

EMDB-3005:
Cytoplasmic ring of the native X. laevis nuclear pore complex.
Method: EM (subtomogram averaging)

EMDB-3006:
Spoke ring and central channel ring of the native X. laevis nuclear pore complex.
Method: EM (subtomogram averaging)

EMDB-3007:
Nucleoplasmic ring of the native X. laevis nuclear pore complex.
Method: EM (subtomogram averaging)

EMDB-3008:
Nuclear envelope of the native X. laevis nuclear pore complex.
Method: EM (subtomogram averaging)

EMDB-3009:
Cytoplasmic ring of the actinomycin D treated X. laevis nuclear pore complex.
Method: EM (subtomogram averaging)

EMDB-3010:
Spoke ring and central channel ring of the actinomycin D treated X. laevis nuclear pore complex.
Method: EM (subtomogram averaging)

EMDB-3011:
Nucleoplasmic ring of the actinomycin D treated X. laevis nuclear pore complex.
Method: EM (subtomogram averaging)

EMDB-3012:
Nuclear envelope of the actinomycin D treated X. laevis nuclear pore complex.
Method: EM (subtomogram averaging)

Source
  • Xenopus laevis (African clawed frog)

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