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| Title | Structural dynamics of ribosome subunit association studied by mixing-spraying time-resolved cryogenic electron microscopy. |
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| Journal, issue, pages | Structure, Vol. 23, Issue 6, Page 1097-1105, Year 2015 |
| Publish date | Jun 2, 2015 |
 Authors | Bo Chen / Sandip Kaledhonkar / Ming Sun / Bingxin Shen / Zonghuan Lu / David Barnard / Toh-Ming Lu / Ruben L Gonzalez / Joachim Frank /  |
| PubMed Abstract | Ribosomal subunit association is a key checkpoint in translation initiation but its structural dynamics are poorly understood. Here, we used a recently developed mixing-spraying, time-resolved, ...Ribosomal subunit association is a key checkpoint in translation initiation but its structural dynamics are poorly understood. Here, we used a recently developed mixing-spraying, time-resolved, cryogenic electron microscopy (cryo-EM) method to study ribosomal subunit association in the sub-second time range. We have improved this method and increased the cryo-EM data yield by tenfold. Pre-equilibrium states of the association reaction were captured by reacting the mixture of ribosomal subunits for 60 ms and 140 ms. We also identified three distinct ribosome conformations in the associated ribosomes. The observed proportions of these conformations are the same in these two time points, suggesting that ribosomes equilibrate among the three conformations within less than 60 ms upon formation. Our results demonstrate that the mixing-spraying method can capture multiple states of macromolecules during a sub-second reaction. Other fast processes, such as translation initiation, decoding, and ribosome recycling, are amenable to study with this method. |
 External links | Structure / PubMed:26004440 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 9.7 - 11.6 Å |
| Structure data |  EMDB-2976:  EMDB-2977:  EMDB-2978: |
| Source |
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Escherichia coli (E. coli)