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TitleStructural organization of an intact phycobilisome and its association with photosystem II.
Journal, issue, pagesCell Res, Vol. 25, Issue 6, Page 726-737, Year 2015
Publish dateMay 22, 2015
AuthorsLeifu Chang / Xianwei Liu / Yanbing Li / Cui-Cui Liu / Fan Yang / Jindong Zhao / Sen-Fang Sui /
PubMed AbstractPhycobilisomes (PBSs) are light-harvesting antennae that transfer energy to photosynthetic reaction centers in cyanobacteria and red algae. PBSs are supermolecular complexes composed of ...Phycobilisomes (PBSs) are light-harvesting antennae that transfer energy to photosynthetic reaction centers in cyanobacteria and red algae. PBSs are supermolecular complexes composed of phycobiliproteins (PBPs) that bear chromophores for energy absorption and linker proteins. Although the structures of some individual components have been determined using crystallography, the three-dimensional structure of an entire PBS complex, which is critical for understanding the energy transfer mechanism, remains unknown. Here, we report the structures of an intact PBS and a PBS in complex with photosystem II (PSII) from Anabaena sp. strain PCC 7120 using single-particle electron microscopy in combination with biochemical and molecular analyses. In the PBS structure, all PBP trimers and the conserved linker protein domains were unambiguously located, and the global distribution of all chromophores was determined. We provide evidence that ApcE and ApcF are critical for the formation of a protrusion at the bottom of PBS, which plays an important role in mediating PBS interaction with PSII. Our results provide insights into the molecular architecture of an intact PBS at different assembly levels and provide the basis for understanding how the light energy absorbed by PBS is transferred to PSII.
External linksCell Res / PubMed:25998682 / PubMed Central
MethodsEM (single particle)
Resolution21.0 - 34.0 Å
Structure data

EMDB-2821:
Negative stain electron microscopy reconstruction of an intact Phycobilisome.
Method: EM (single particle) / Resolution: 21.0 Å

EMDB-2822:
Negative stain electron microscopy reconstruction of an intact Phycobilisome in complex with photosystem II.
Method: EM (single particle) / Resolution: 34.0 Å

Source
  • Anabaena (bacteria)

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