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TitleConformational changes leading to T7 DNA delivery upon interaction with the bacterial receptor.
Journal, issue, pagesJ Biol Chem, Vol. 290, Issue 16, Page 10038-10044, Year 2015
Publish dateApr 17, 2015
AuthorsVerónica A González-García / Mar Pulido-Cid / Carmela Garcia-Doval / Rebeca Bocanegra / Mark J van Raaij / Jaime Martín-Benito / Ana Cuervo / José L Carrascosa /
PubMed AbstractThe majority of bacteriophages protect their genetic material by packaging the nucleic acid in concentric layers to an almost crystalline concentration inside protein shells (capsid). This highly ...The majority of bacteriophages protect their genetic material by packaging the nucleic acid in concentric layers to an almost crystalline concentration inside protein shells (capsid). This highly condensed genome also has to be efficiently injected into the host bacterium in a process named ejection. Most phages use a specialized complex (often a tail) to deliver the genome without disrupting cell integrity. Bacteriophage T7 belongs to the Podoviridae family and has a short, non-contractile tail formed by a tubular structure surrounded by fibers. Here we characterize the kinetics and structure of bacteriophage T7 DNA delivery process. We show that T7 recognizes lipopolysaccharides (LPS) from Escherichia coli rough strains through the fibers. Rough LPS acts as the main phage receptor and drives DNA ejection in vitro. The structural characterization of the phage tail after ejection using cryo-electron microscopy (cryo-EM) and single particle reconstruction methods revealed the major conformational changes needed for DNA delivery at low resolution. Interaction with the receptor causes fiber tilting and opening of the internal tail channel by untwisting the nozzle domain, allowing release of DNA and probably of the internal head proteins.
External linksJ Biol Chem / PubMed:25697363 / PubMed Central
MethodsEM (single particle)
Resolution20.0 Å
Structure data

EMDB-2717:
Electron cryo-microscopy of T7 bacteriophage tail after DNA ejection
Method: EM (single particle) / Resolution: 20.0 Å

Source
  • Enterobacteria phage T7 (virus)

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