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TitleLemon-shaped halo archaeal virus His1 with uniform tail but variable capsid structure.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 112, Issue 8, Page 2449-2454, Year 2015
Publish dateFeb 24, 2015
AuthorsChuan Hong / Maija K Pietilä / Caroline J Fu / Michael F Schmid / Dennis H Bamford / Wah Chiu /
PubMed AbstractLemon-shaped viruses are common in nature but so far have been observed to infect only archaea. Due to their unusual shape, the structures of these viruses are challenging to study and therefore ...Lemon-shaped viruses are common in nature but so far have been observed to infect only archaea. Due to their unusual shape, the structures of these viruses are challenging to study and therefore poorly characterized. Here, we have studied haloarchaeal virus His1 using cryo-electron tomography as well as biochemical dissociation. The virions have different sizes, but prove to be extremely stable under various biochemical treatments. Subtomogram averaging of the computationally extracted virions resolved a tail-like structure with a central tail hub density and six tail spikes. Inside the tail there are two cavities and a plug density that separates the tail hub from the interior genome. His1 most likely uses the tail spikes to anchor to host cells and the tail hub to eject the genome, analogous to classic tailed bacteriophages. Upon biochemical treatment that releases the genome, the lemon-shaped virion transforms into an empty tube. Such a dramatic transformation demonstrates that the capsid proteins are capable of undergoing substantial quaternary structural changes, which may occur at different stages of the virus life cycle.
External linksProc Natl Acad Sci U S A / PubMed:25675521 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution20.0 - 28.0 Å
Structure data

EMDB-6220:
Electron cryo-tomography of lemon-shaped virus His1
Method: EM (subtomogram averaging)

EMDB-6221:
Refined structure of lemon-shaped virus His1 using Cryo-ET subtomogram averaging
Method: EM (subtomogram averaging) / Resolution: 28.0 Å

EMDB-6222:
Six-fold averaged structure of lemon-shaped virus His1 using Cryo-ET subtomogram averaging
Method: EM (subtomogram averaging) / Resolution: 20.0 Å

EMDB-6223:
Subtomogram averaging of the empty tube of lemon-shaped archaeal virus His1
Method: EM (subtomogram averaging)

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